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Plant and Cell Physiology, 2002, Vol. 43, No. 8 932-938
© 2002 Oxford University Press

Protection of the Oxygen-Evolving Machinery by the Extrinsic Proteins of Photosystem II is Essential for Development of Cellular Thermotolerance in Synechocystis sp. PCC 6803

Aiko Kimura1,2, Julian J. Eaton-Rye3, Eugene H. Morita1,2,4, Yoshitaka Nishiyama1,2,5 and Hidenori Hayashi1,2

1 Department of Chemistry, Faculty of Science, Ehime University, Matsuyama, 790-8577 Japan
2 Satellite Venture Business Laboratory, Ehime University, Matsuyama, 790-8577 Japan
3 Department of Biochemistry, University of Otago, P.O. Box 56, Dunedin, New Zealand

The oxygen-evolving machinery of photosystem II in cyanobacteria is associated with three extrinsic proteins: the manganese-stabilizing protein, cytochrome c550, and PsbU. To elucidate the effect of the presence of these extrinsic proteins on the stabilization of the oxygen-evolving machinery against high-temperature stress, we inactivated the genes for these proteins individually in Synechocystis sp. PCC 6803 by targeted mutagenesis. The thermal stability of the oxygen-evolving machinery decreased in all mutated cells but the extent of the susceptibility to heat inactivation varied between the photosystems lacking the different extrinsic proteins. Cells that lacked either the manganese-stabilizing protein or cytochrome c550 were unable to enhance the thermal stability of the oxygen-evolving machinery and, moreover, failed to increase cellular thermotolerance when grown at moderately high temperatures. Our findings indicate that the three extrinsic proteins stabilize the oxygen-evolving machinery independently against high-temperature stress and that the thermal stability of the machinery influences cellular thermotolerance.

4 Present address: Center for Gene Research, Ehime University, Matsuyama, 790-8566 Japan.

5 Corresponding author: E-mail, nishiyama@chem.sci.ehime-u.ac.jp; Fax, +81-89-927-9611.


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