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Plant and Cell Physiology, 2002, Vol. 43, No. 6 604-613
© 2002 Oxford University Press

Cell-Cycle Dependent Dynamic Change of 26S Proteasome Distribution in Tobacco BY-2 Cells

Yuki Yanagawa1,8, Seiichiro Hasezawa2, Fumi Kumagai2, Masayoshi Oka1, Masahiro Fujimuro3, Tae Naito1, Taro Makino1, Hideyoshi Yokosawa3, Keiji Tanaka4,5, Atsushi Komamine6, Junji Hashimoto5,7, Takahide Sato1 and Hiroki Nakagawa1,9

1 Department of Bioproduction Science, Faculty of Horticulture, Chiba University, Matsudo, Matsudo, Chiba, 271-8510 Japan
2 Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, 113-0033 Japan
3 Department of Biochemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo, 060-0812 Japan
4 The Tokyo Metropolitan Institute of Medical Science, Honkomagome, Bunkyo-ku, Tokyo, 113-8613 Japan
5 Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (JST), Chiyoda-ku, Tokyo, 101-0062 Japan
6 The Research Institute of Evolutionary Biology, Kamiyoga, Setagaya-ku, Tokyo, 158-0098 Japan
7 National Institute of Agrobiological Sciences, Kannondai, Tsukuba, Ibaraki, 305-8602 Japan

The 26S proteasome is known to play pivotal roles in cell-cycle progression in various eukaryotic cells; however, little is known about its role in higher plants. Here we report that the subcellular distribution of the 26S proteasome is dynamically changed in a cell-cycle dependent manner in tobacco BY-2 cells as determined by immunostaining with anti-Rpn10 (a regulatory PA700 subunit) and anti-20S catalytic proteasome antibodies. The 26S proteasome was found to localize not only in nuclear envelopes and mitotic spindles but also in preprophase bands (PPBs) and phragmoplasts appearing in G2 and M phases, respectively. MG132, a proteasome inhibitor, exclusively caused cell-cycle arrest not only at the metaphase but also the early stage of PPB formation at the G2 phase and the collapse of the phragmoplast, which seems to be closely related to proteasome distribution in the cells.

8 Present address: Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520-8104, U.S.A.

9 Corresponding author: E-mail, nakagawa@midori.h.chiba-u.ac.jp; Fax, +81-47-308-8862.


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