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Plant and Cell Physiology, 2002, Vol. 43, No. 5 540-548
© 2002 Oxford University Press

Random Mutagenesis Targeted to the psbAII Gene of Synechocystis sp. PCC 6803 to Identify Functionally Important Residues in the D1 Protein of the Photosystem II Reaction Center

Akihiro Yamasato1,3,4, Tomoe Kamada1,4,5 and Kimiyuki Satoh1,2,6,7

1 Graduate School of Natural Science and Technology, Okayama University, Okayama, 700-8530 Japan 2 Department of Biology, Faculty of Science, Okayama University, Okayama, 700-8530 Japan

More than one hundred mutants of Synechocystis sp. PCC 6803 impaired in photoautotrophic growth were generated by in vitro random PCR mutagenesis targeted to a region of the psbAII gene corresponding to a 210 amino acid (Ser148–Ala357) segment of the D1 protein. The 90 random mutants that could translate the full-length D1 protein carried 1–9 (on average 3.0) amino acid substitutions in the targeted region. Mutations were often found in the obligate photoheterotrophic strains at specific residues that have been reported or speculated to be important in the function of PSII, such as Y161, H198, H272, E333 and H337. This verifies the usefulness of the present method to identify functionally important residues in PSII. Other residues that were often mutated in the strains with impaired photoautotrophy included non-charged residues around the lumenal edges of transmembrane helices C, D and E, such as I192 and N296. Eleven mutants carried a single-point mutation in residues, such as Q165, Q187, W278, A294 and N298, and these identified the functional importance of these residues, most of which were on the donor side of PSII. A preliminary characterization of some of the mutants obtained in this study is provided.

3 Present address: Institute of Low Temperature Science, Hokkaido University, Sapporo, 060-0819 Japan.

4 The first two authors contributed equally to this work.

5 Present address: National Institute for Basic Biology, Okazaki, 444-8585 Japan.

6 Present address: Department of Plant Biology and Center for the Study of Early Events in Photosynthesis, Arizona State University, Tempe, AZ 85287-1601, U.S.A.

7 Corresponding author: E-mail, kimiyuki@cc.okayama-u.ac.jp.


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