Plant and Cell Physiology, 2002, Vol. 43, No. 4 402-410
© 2002 Oxford University Press
Regulation of Gene Expression by Low Levels of Ultraviolet-B Radiation in Pisum sativum: Isolation of Novel Genes by Suppression Subtractive Hybridisation
1 Biochemistry and Biophysics, Department of Chemistry, Göteborg University, P.O. Box 462, S-405 30 Göteborg, Sweden 2 Department of Natural Sciences, Örebro University, S-701 82 Örebro, Sweden
Suppression subtractive hybridisation was used to isolate genes differentially regulated by low levels (UV-BBE,300 0.13 W m–2) of ultraviolet-B radiation (UV-B; 290–320 nm) in Pisum sativum. Six genes were regulated, two of which were novel. The mRNA levels for these two (PsTSDC and PsUOS1) were increased and depressed by UV-B treatment, respectively. Domains in the PsTSDC translation product was similar to TIR (Toll-Interleukin-1 receptor-similar) domains and a NB-ARC domain (nucleotide-binding domain in APAF-1, R gene products and CED-4). The PsUOS1 translation product was similar to an open reading frame in Arabidopsis. Genes encoding embryo-abundant protein (PsEMB) and S-adenosyl-L-methionine synthase (PsSAMS) were induced by UV-B, whereas the transcript levels for genes encoding sucrose transport protein (PsSUT) or ribulose-5-phosphate 3-epimerase (PsR5P3E) were decreased. These regulation patterns are novel, and the PsEMB and PsR5P3E sequences are reported for the first time. The stress-specificity of regulation of these genes were tested by ozone fumigation (100 ppb O3). Qualitatively, the similarity of expression after both UV-B and ozone exposure suggests that, for these genes, similar stress-response pathways are in action.
3 Present address: Institute of Biotechnology, P.O. Box 56, FIN-00014, University of Helsinki, Finland.
4 Corresponding author: E-mail, ake.strid@nat.oru.se; Fax, +46-19-303566.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Schult, K. Meierhoff, S. Paradies, T. Toller, P. Wolff, and P. Westhoff The Nuclear-Encoded Factor HCF173 Is Involved in the Initiation of Translation of the psbA mRNA in Arabidopsis thaliana PLANT CELL, April 1, 2007; 19(4): 1329 - 1346. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Ballare Stress Under the Sun: Spotlight on Ultraviolet-B Responses Plant Physiology, August 1, 2003; 132(4): 1725 - 1727. [Full Text] [PDF]
|
|
|
|
|
|
M. M. Izaguirre, A. L. Scopel, I. T. Baldwin, and C. L. Ballare Convergent Responses to Stress. Solar Ultraviolet-B Radiation and Manduca sexta Herbivory Elicit Overlapping Transcriptional Responses in Field-Grown Plants of Nicotiana longiflora Plant Physiology, August 1, 2003; 132(4): 1755 - 1767. [Abstract] [Full Text] [PDF]
|
|