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Plant and Cell Physiology, 2002, Vol. 43, No. 4 379-383
© 2002 Oxford University Press

Effects of Light and Low Oxygen Tension on Pigment Biosynthesis in Halobacterium salinarum, Revealed by a Novel Method to Quantify Both Retinal and Carotenoids

Wael S. M. El-Sayed1, Shinichi Takaichi2, Haruo Saida3, Masahiro Kamekura4, Mohamed Abu-Shady1, Humitake Seki5 and Tomohiko Kuwabara5,6

1 Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt 2 Biological Laboratory, Nippon Medical School, Kawasaki, 211-0063 Japan 3 Faculty of Regional Science, Toyama University of International Studies, Toyama, 930-1292 Japan 4 Noda Institute for Scientific Research, Noda, 278-0037 Japan 5 Institute of Biological Sciences, University of Tsukuba, Tsukuba, 305-8572 Japan

A novel method for analyzing halobacterial pigments was developed, in which retinal was liberated from halobacterial rhodopsins as retinal oxime by hydroxylamine, ethyl ß-apo-8'-carotenoate was introduced as an internal standard, and the pigments including bacterioruberin and ß-carotene were analyzed by HPLC at the same time. With this method, we revealed that light enhances the biosynthesis of bacterioruberin and the conversion of ß-carotene to retinal, but does not affect ß-carotene biosynthesis in Halobacterium salinarum strain Oyon Moussa-16. Low oxygen tension given in the light brought a slight increase in retinal accumulation, although its biosynthesis from ß-carotene is an oxygenation reaction. This paradox could be explained by the increase in ß-carotene biosynthesis.

6 Corresponding author: E-mail, kuwabara@biol.tsukuba.ac.jp; Fax, +81-298-53-6614.


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