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Plant and Cell Physiology, 2002, Vol. 43, No. 2 207-216
© 2002 Oxford University Press

Aluminum-Induced Rapid Changes in the Microtubular Cytoskeleton of Tobacco Cell Lines

Katerina Schwarzerová1,3, Sylva Zelenková1, Peter Nick2 and Zdenek Opatrny1

1 Department of Plant Physiology, Faculty of Science, Charles University, Vinicná 5, 12844 Prague, Czech Republic 2 Institut für Biologie II, Albert-Ludwigs University, Schänzlestr. 1, Freiburg, Germany

Aluminum (Al) is a major factor that limits plant growth in acid soils. It causes a cessation of root growth and changes in root morphology suggesting a role of the root cytoskeleton as a target of Al-toxicity. Here we report a rapid effect of Al on the microtubular cytoskeleton of the suspension tobacco cell lines BY-2 and VBI-0. Viability studies showed that the cells were more sensitive to Al during exponential phase as compared to stationary cells. During the first hours of exposure, Al induced the formation of additional bundles of cortical microtubules (cMTs), whereas the thickness of the individual bundles decreased. Prolonged exposure resulted in disorientation of cMTs. These changes of cMTs preceded the decrease of cell viability by several hours and were accompanied by an increase in the levels of {alpha}-tubulin (in its tyrosinated form) and elements of the tubulin-folding chaperone CCT. These findings suggest that the microtubular cytoskeleton is one of the early targets of Al toxicity.

3 Corresponding author: E-mail, schwarze@prfdec.natur.cuni.cz; Fax, +420-2-2195-3306.


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