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Plant and Cell Physiology, 2002, Vol. 43, No. 11 1386-1389
© 2002 Oxford University Press

Short Communications

A Sensitive and Rapid Bioassay of Homogalacturonan Synthase Using 2-Aminobenzamide-Labeled Oligogalacturonides

Tadashi Ishii1

Forestry and Forest Products Research Institute, P. O. Box 16, Tsukuba Norin Kenkyu Danchi-nai, Ibaraki, 305-8687 Japan

Abstract

Polygalacturonate 4-{alpha}-galacturonosyltransferase (GalA T) activity was detected in the microsomal fraction isolated from pumpkin (Cucurbia moschata Duchesne, cv. Tokyou-Kabocha) seedlings using UDP-GalA and 2-aminobenzamide (2AB)-labeled oligogalacturonides. A 2AB-labeled undecagalacturonide was elongated by the attachment of galacturonic acid (GalA) residues to give 2AB-labeled oligogalacturonides with a degree of polymerization (DP) beetween 12 and 17. Exogenous 2AB-labeled oligogalacturonide acceptors with a DP >3 are effective acceptor molecules for pumpkin GalA T.

Footnotes

1 E-mail, tishii@ffpri.affrc.go.jp; Fax, +81-298-74-3720.


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