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Plant and Cell Physiology, 2002, Vol. 43, No. 11 1314-1322
© 2002 Oxford University Press

Transcriptional Response of Soybean Suspension-cultured Cells Induced by Nod Factors Obtained from Bradyrhizobium japonicum USDA110

Tsuneo Hakoyama1, Tadashi Yokoyama2,3, Hiroshi Kouchi2, Ken-ichi Tsuchiya2,4, Hisatoshi Kaku2 and Yasuhiro Arima1,5

1 Faculty of Agriculture, Tokyo University of Agriculture and Technology Fuchu, Tokyo, 183-8509 Japan
2 National Institute of Agro-Biological Sciences, Tsukuba Ibaraki, 305-8602 Japan

Genes responding to Nod factors were picked up by the application of a differential display method for soybean suspension-cultured cells. Forty-five cDNA fragments derived from such genes were detected. Seven fragments (ssc1–ssc7) were successfully cloned. The putative product of genes corresponding to ssc1 was estimated to be a disease-resistance protein relating to the induction of the plant defense response against pathogens, and that corresponding to ssc7 was a sucrose transporter. Amino acid sequences deduced from full-length cDNA corresponding to ssc2 and ssc4 were investigated, and it was shown that these polypeptides were equipped with a leucine zipper motif and with phosphorylation sites that were targeted by tyrosin kinase and cAMP-dependent protein kinase, respectively. In a differential display experiment, the transcriptional levels of three genes corresponding to ssc2, ssc3 and ssc5 were estimated to be up-regulated at 6 h after initiation of the treatment and the remaining four were estimated to be down-regulated. However, transcription of the genes corresponding to all ssc was clearly repressed within 2 h after initiation of the treatment. Five of them were restored to their transcriptional level 6 h after initiation of the treatment, although the others were repressed throughout the experimental period.

3 Present address: Tokyo University of Agriculture and Technology, Saiwaicho 3-5-8, Fuchu, Tokyo, 183-8509, Japan.

4 Present address: National Institute for Agro-Environmental Sciences, Kannondai 3-1-3, Tsukuba, Ibaraki, 305-8604 Japan.

5 Corresponding author: E-mail, hakoyama@cc.tuat.ac.jp; Fax, +81-42-367-5677.


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