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Plant and Cell Physiology, 2002, Vol. 43, No. 1 91-98
© 2002 Oxford University Press

A Pharmacological Approach to Test the Diffusible Signal Activity of Reactive Oxygen Intermediates in Elicitor-Treated Tobacco Leaves

Laurent Costet1, Stephan Dorey2, Bernard Fritig and Serge Kauffmann3

Institut de Biologie Moléculaire des Plantes du C.N.R.S., Université Louis Pasteur, 12 rue du Général Zimmer, F-67084 Strasbourg, France

The capacity of H2O2, the most stable of the reactive oxygen species (ROI), to diffuse freely across biological membranes and to signal gene expression suggests that H2O2 could function as a short-lived second messenger diffusing from cell to cell. We tested this hypothesis in tobacco plants treated with a glycoprotein elicitor. Applied at 50 nM, it induces H2O2 accumulation and the hypersensitive response restricted to the infiltrated zone 1 tissue. Stimulation of a set of defense responses also occurs in the surrounding zone 2 tissue without diffusion of the elicitor. ROI levels in zone 1 were modulated using N-acetyl-L-cysteine (NAC) as a ROI scavenger and Rose Bengal (RB) as a ROI generator. We found that ROI appeared to act as signalling intermediates in pathways leading to salicylic acid accumulation, to PR1, PR5 and 3-hydroxy-3-methylglutarylCoA reductase expression in glycoprotein-treated zone 1 tissues. Compared to the treatment with the elicitor alone, co-infiltration of the glycoprotein and NAC increased the surface of zone 2 showing PR1 and O-methyltransferase expression. Application of RB had the opposite effect. The data suggest that, in our system, ROI did not act as a cell-to-cell diffusible signal to activate PR protein and O-methyltransferase expression in zone 2.

1 Present address: CIRAD-CA, Station de la Bretagne, Laboratoire de Phytopathologie, BP 20, F-97408 St Denis, France.

2 Present address: The Sainsbury Laboratory, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, U.K.

3 Corresponding author: E-mail, serge.kauffmann@ibmp-ulp.u-strasbg.fr; Fax, +33-388-61-4442.


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