Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (26)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kaku, T.
Right arrow Articles by Hoson, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kaku, T.
Right arrow Articles by Hoson, T.
Agricola
Right arrow Articles by Kaku, T.
Right arrow Articles by Hoson, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 2002, Vol. 43, No. 1 21-26
© 2002 Oxford University Press

Action of Xyloglucan Hydrolase within the Native Cell Wall Architecture and Its Effect on Cell Wall Extensibility in Azuki Bean Epicotyls

Tomomi Kaku1, Akira Tabuchi1,2, Kazuyuki Wakabayashi, Seiichiro Kamisaka3 and Takayuki Hoson4

Department of Biological Sciences, Graduate School of Science, Osaka City University, Sugimoto, Sumiyoshi-ku, Osaka, 558-8585 Japan

Xyloglucan hydrolase (XGH) has recently been purified from the cell wall of azuki bean (Vigna angularis Ohwi et Ohashi) epicotyls as a new type of xyloglucan-degrading enzyme [Tabuchi et al. (2001) Plant Cell Physiol. 42: 154]. In the present study, the effects of XGH on the mechanical properties of the cell wall and on the level and the molecular size of xyloglucans within the native wall architecture were examined in azuki bean epicotyls. When the epidermal tissue strips from the growing regions of azuki bean epicotyls were incubated with XGH, the mechanical extensibility of the cell wall dramatically increased. XGH exogenously applied to cell wall materials (homogenates) or epidermal tissue strips decreased the amount of xyloglucans via the solubilization of the polysaccharides. Also, XGH substantially decreased the molecular mass of xyloglucans in both materials. These results indicate that XGH is capable of hydrolyzing xyloglucans within the native cell wall architecture and thereby increasing the cell wall extensibility in azuki bean epicotyls.

1 These authors contributed equally to this work.

2 Present address: Research Institute for Bioresources, Okayama University, Chuo, Kurashiki, 710-0046 Japan.

3 Present address: Department of Biology, Faculty of Science, Toyama University, Gofuku, Toyama, 930-8555 Japan.

4 Corresponding author: E-mail, hoson@sci.osaka-cu.ac.jp; Fax, +81-6-6605-2577.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
R. Sasidharan, C.C. Chinnappa, L. A.C.J. Voesenek, and R. Pierik
The Regulation of Cell Wall Extensibility during Shade Avoidance: A Study Using Two Contrasting Ecotypes of Stellaria longipes
Plant Physiology, November 1, 2008; 148(3): 1557 - 1569.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
V. Genovesi, S. Fornale, S. C. Fry, K. Ruel, P. Ferrer, A. Encina, F.-M. Sonbol, J. Bosch, P. Puigdomenech, J. Rigau, et al.
ZmXTH1, a new xyloglucan endotransglucosylase/hydrolase in maize, affects cell wall structure and composition in Arabidopsis thaliana
J. Exp. Bot., March 3, 2008; (2008) ern013v2.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
V. S. T. Van Sandt, D. Suslov, J.-P. Verbelen, and K. Vissenberg
Xyloglucan Endotransglucosylase Activity Loosens a Plant Cell Wall
Ann. Bot., December 1, 2007; 100(7): 1467 - 1473.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
F. Z. Zhang, C. Wagstaff, A. M. Rae, A. K. Sihota, C. W. Keevil, S. D. Rothwell, G. J. J. Clarkson, R. W. Michelmore, M. J. Truco, M. S. Dixon, et al.
QTLs for shelf life in lettuce co-locate with those for leaf biophysical properties but not with those for leaf developmental traits
J. Exp. Bot., April 1, 2007; 58(6): 1433 - 1449.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
N. O'LOONEY and S. C. FRY
Oxaziclomefone, a New Herbicide, Inhibits Wall Expansion in Maize Cell-cultures without Affecting Polysaccharide Biosynthesis, Xyloglucan Transglycosylation, Peroxidase Action or Apoplastic Ascorbate Oxidation
Ann. Bot., November 1, 2005; 96(6): 1097 - 1107.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Guan and E. A. Nothnagel
Binding of Arabinogalactan Proteins by Yariv Phenylglycoside Triggers Wound-Like Responses in Arabidopsis Cell Cultures
Plant Physiology, July 1, 2004; 135(3): 1346 - 1366.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Z. Minic, C. Rihouey, C. T. Do, P. Lerouge, and L. Jouanin
Purification and Characterization of Enzymes Exhibiting {beta}-D-Xylosidase Activities in Stem Tissues of Arabidopsis
Plant Physiology, June 1, 2004; 135(2): 867 - 878.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Kaku, A. Tabuchi, K. Wakabayashi, and T. Hoson
Xyloglucan Oligosaccharides Cause Cell Wall Loosening by Enhancing Xyloglucan Endotransglucosylase/Hydrolase Activity in Azuki Bean Epicotyls
Plant Cell Physiol., January 15, 2004; 45(1): 77 - 82.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
J. K. C. Rose, J. Braam, S. C. Fry, and K. Nishitani
The XTH Family of Enzymes Involved in Xyloglucan Endotransglucosylation and Endohydrolysis: Current Perspectives and a New Unifying Nomenclature
Plant Cell Physiol., December 15, 2002; 43(12): 1421 - 1435.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.