Plant and Cell Physiology, 2001, Vol. 42, No. 9 900-905
© 2001 Oxford University Press
A Putative Two Pore Channel AtTPC1 Mediates Ca2+ Flux in Arabidopsis Leaf Cells
1 Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya, 464-8601 Japan 2 Nagoya University Bioscience Center, Nagoya University, Chikusa-ku, Nagoya, 464-8601 Japan 3 Department of Biology, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, U.S.A.
The gene encoding voltage-gated channel with high affinity for Ca2+ permeation has not been cloned from plants. In the present study, we isolated a full-length cDNA encoding a putative Ca2+ channel (AtTPC1) from Arabidopsis. AtTPC1 has two conserved homologous domains, both of which contain six transmembrane segments (S1–S6) and a pore loop (P) between S5 and S6 in each domain, and has the highest homology with the two pore channel TPC1 recently cloned from rat. The overall structure is similar to the half of the general structure of 
-subunits of voltage-activated Ca2+ channels from animals. AtTPC1 rescued the Ca2+ uptake activity of a yeast mutant cch1. Sucrose-induced luminescence, which reflects a cytosolic free Ca2+ increase in aequorin-expressing Arabidopsis leaves, was enhanced by overexpression of AtTPC1 and suppressed by antisense expression of it. Sucrose-H+ symporters AtSUC1 and 2, which depolarize membrane potential of cells receiving sucrose, also depressed a Ca2+ increase by their antisense expression. These results suggest that AtTPC1 mediates a voltage-activated Ca2+ influx in Arabidopsis leaf cells.
4 Corresponding author: E-mail, h44787a@nucc.cc.nagoya-u.ac.jp; Fax, +81-52-789-5206.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  G. Bonaventure, A. Gfeller, V. M. Rodriguez, F. Armand, and E. E. Farmer The fou2 Gain-of-Function Allele and the Wild-Type Allele of Two Pore Channel 1 Contribute to Different Extents or by Different Mechanisms to Defense Gene Expression in Arabidopsis Plant Cell Physiol., December 1, 2007; 48(12): 1775 - 1789. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. I. Pottosin and G. Schonknecht Vacuolar calcium channels J. Exp. Bot., May 1, 2007; 58(7): 1559 - 1569. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-J. Wang, J.-N. Yu, T. Chen, Z.-G. Zhang, Y.-J. Hao, J.-S. Zhang, and S.-Y. Chen Functional analysis of a putative Ca2+ channel gene TaTPC1 from wheat J. Exp. Bot., December 1, 2005; 56(422): 3051 - 3060. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-l. Shang, L.-g. Ma, H.-l. Zhang, R.-r. He, X.-c. Wang, S.-j. Cui, and D.-y. Sun Ca2+ Influx into Lily Pollen Grains Through a Hyperpolarization-activated Ca2+-permeable Channel Which Can be Regulated by Extracellular CaM Plant Cell Physiol., April 1, 2005; 46(4): 598 - 608. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Nagata, S. Iizumi, K. Satoh, H. Ooka, J. Kawai, P. Carninci, Y. Hayashizaki, Y. Otomo, K. Murakami, K. Matsubara, et al. Comparative Analysis of Plant and Animal Calcium Signal Transduction Element Using Plant Full-Length cDNA Data Mol. Biol. Evol., October 1, 2004; 21(10): 1855 - 1870. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kurusu, Y. Sakurai, A. Miyao, H. Hirochika, and K. Kuchitsu Identification of a Putative Voltage-Gated Ca2+-permeable Channel (OsTPC1) Involved in Ca2+ Influx and Regulation of Growth and Development in Rice Plant Cell Physiol., June 15, 2004; 45(6): 693 - 702. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. M. VOLK, L. J. GOSS, and V. R. FRANCESCHI Calcium Channels are Involved in Calcium Oxalate Crystal Formation in Specialized Cells of Pistia stratiotes L. Ann. Bot., June 1, 2004; 93(6): 741 - 753. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hashimoto, M. Saito, H. Matsuoka, K. Iida, and H. Iida Functional Analysis of a Rice Putative Voltage-Dependent Ca2+ Channel, OsTPC1, Expressed in Yeast Cells Lacking its Homologous Gene CCH1 Plant Cell Physiol., April 15, 2004; 45(4): 496 - 500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kadota, T. Goh, H. Tomatsu, R. Tamauchi, K. Higashi, S. Muto, and K. Kuchitsu Cryptogein-Induced Initial Events in Tobacco BY-2 Cells: Pharmacological Characterization of Molecular Relationship among Cytosolic Ca2+ Transients, Anion Efflux and Production of Reactive Oxygen Species Plant Cell Physiol., February 15, 2004; 45(2): 160 - 170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. WHITE and M. R. BROADLEY Calcium in Plants Ann. Bot., October 1, 2003; 92(4): 487 - 511. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Sanders, J. Pelloux, C. Brownlee, and J. F. Harper Calcium at the Crossroads of Signaling PLANT CELL, May 1, 2002; 14(90001): S401 - 417. [Full Text] [PDF]
|
|
|
|
 |
|
 M. Bonilla and K. W. Cunningham Calcium Release and Influx in Yeast: TRPC and VGCC Rule Another Kingdom Sci. Signal., April 9, 2002; 2002(127): pe17 - pe17. [Abstract] [Full Text] [PDF]
|
|