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Plant and Cell Physiology, 2001, Vol. 42, No. 4 414-418
© 2001 Oxford University Press

The Establishment of Conditions to Efficiently Screen Photosynthesis-Deficient Mutants of Synechocystis sp. PCC 6803 by Nitrofurantoin Treatment

Akihiro Yamasato1 and Kimiyuki Satoh ,1,2,3

1 Graduate School of Natural Science and Technology, Okayama University, Okayama, 700-8530 Japan 2 Department of Biology, Faculty of Science, Okayama University, Okayama, 700-8530 Japan

A method based on the susceptibility of photosynthetic organisms to nitrofurantoin under illumination was established to screen mutants of Synechocystis sp. PCC 6803 deficient in the function of photosystem II, which were created by random PCR mutagenesis targeted to the psbAII gene coding for the D1 protein of the photosystem II reaction center. In this method, cyanobacterial colonies on a nitrocellulose membrane on a BG11 agar plate were treated with nitrofurantoin at 1.0 mM under white light at 40 µE m–2 s–1 for 2 h, and then kept under normal conditions without nitrofurantoin so that surviving cells could grow. This method was also shown to be useful for screening mutants deficient in the function of photosystem I.

3 Corresponding author: E-mail, kimiyuki@cc.okayama-u.ac.jp; Fax, +81-86-251-7877.


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A. Yamasato, T. Kamada, and K. Satoh
Random Mutagenesis Targeted to the psbAII Gene of Synechocystis sp. PCC 6803 to Identify Functionally Important Residues in the D1 Protein of the Photosystem II Reaction Center
Plant Cell Physiol., May 15, 2002; 43(5): 540 - 548.
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