Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (19)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Ueda, A.
Right arrow Articles by Takabe, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ueda, A.
Right arrow Articles by Takabe, T.
Agricola
Right arrow Articles by Ueda, A.
Right arrow Articles by Takabe, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 2001, Vol. 42, No. 11 1282-1289
© 2001 Oxford University Press

Functional Analysis of Salt-Inducible Proline Transporter of Barley Roots

Akihiro Ueda1, Weiming Shi2, Kazutsuka Sanmiya3, Mariko Shono3 and Tetsuko Takabe2,4

1 Bioscience Center, Nagoya University, Chikusa, Nagoya, 464-8601 Japan 2 Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601 Japan 3 Japan International Research Center for Agricultural Sciences, Ishigaki, 907-0002 Japan

We cloned a cDNA encoding Hordeum vulgare Proline Transporter (HvProT) from salt-stressed barley roots by differential display. HvProT was 2,161 bp long and had an open reading frame encoding 450 amino acids. The deduced amino acid sequence of HvProT was similar to those of proline transporter proteins of rice (65.7%), Arabidopsis (57.7%) and tomato (42.0%). Northern blot analysis showed that the transcript level of HvProT was induced in roots at 30 min after 200 mM NaCl treatment and its peak was observed at 3 h. However, the transcript level was very low in leaves and did not increase by salt stress. The expression level of {Delta}1-pyrroline-5-carboxylate synthetase (P5CS), encoding a key enzyme of proline synthesis, was induced later than HvProT by salt stress. A transport assay using a yeast with mutation in proline uptake revealed that HvProT was a transporter with high affinity for L-proline (Km = 25 µM). HvProT was found to be a unique transporter with high affinity for L-proline. Since its transport activity was dependent on the pH gradient, HvProT was suggested to be a H+/amino acid symporter. In situ hybridization analysis showed that the HvProT mRNA was strongly expressed in root cap cells under salt stress. HvProT might play an important role in the transport of proline to root tip region urgently upon salt stress.

4 Corresponding author: E-mail, h44854a@nucc.cc.nagoya-u.ac.jp; Fax, +81-52-789-5209.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
A. Meyer, S. Eskandari, S. Grallath, and D. Rentsch
AtGAT1, a High Affinity Transporter for {gamma}-Aminobutyric Acid in Arabidopsis thaliana
J. Biol. Chem., March 17, 2006; 281(11): 7197 - 7204.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. A. Cuin and S. Shabala
Exogenously Supplied Compatible Solutes Rapidly Ameliorate NaCl-induced Potassium Efflux from Barley Roots
Plant Cell Physiol., December 1, 2005; 46(12): 1924 - 1933.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Grallath, T. Weimar, A. Meyer, C. Gumy, M. Suter-Grotemeyer, J.-M. Neuhaus, and D. Rentsch
The AtProT Family. Compatible Solute Transporters with Similar Substrate Specificity But Differential Expression Patterns
Plant Physiology, January 1, 2005; 137(1): 117 - 126.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Ueda, A. Kathiresan, M. Inada, Y. Narita, T. Nakamura, W. Shi, T. Takabe, and J. Bennett
Osmotic stress in barley regulates expression of a different set of genes than salt stress does
J. Exp. Bot., October 1, 2004; 55(406): 2213 - 2218.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.