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Plant and Cell Physiology, 2000, Vol. 41, No. 9 1067-1071
© 2000 Oxford University Press

Quantification of Ca2+/H+ Antiporter VCAX1p in Vacuolar Membranes and Its Absence in Roots of Mung Bean

Hanayo Ueoka-Nakanishi1 and Masayoshi Maeshima1,2

1 Laboratory of Biochemistry, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601 Japan

Vacuolar Ca2+/H+ antiporter VCAX1p, which contributes to the Ca2+ accumulation into vacuoles, was quantified by immunochemistry. The antiporter content in vacuolar membranes was 0.14 and 1.1 µg mg–1 of membrane protein for hypocotyls and epicotyls, respectively. The calculated turnover number was 120 s–1. Roots lacked the antiporter protein and the transcript.

2 Corresponding author: E-mail, maeshima@agr.nagoya-u.ac.jp; Fax, +81-52-789-4094.


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J. K. Pittman, C. S. Sreevidya, T. Shigaki, H. Ueoka-Nakanishi, and K. D. Hirschi
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