Plant and Cell Physiology, 1996, Vol. 37, No. 1 49-59
© 1996
Efficient Promoter Cassettes for Enhanced Expression of Foregin Genes in Dicotyledonous and Monocotyledonous Plants
1 National Institute of Agrobiological Resources Tsukuba, Ibaraki, 305 Japan
2 Miyagi Prefectural Agricultural Research Center Natori, Miyagi, 981-12 Japan
3 Ibaraki Horticultural Research Station Iwama, Ibaraki, 311-32 Japan
4 Kagoshima Biotechnology Institute Kushira, Kagoshima, 893-16 Japan
5 Tohoku National Agricultural Experimental Station Omagari, Akita, 014-01 Japan
6 National Agriculture Research Center Tsukuba, Ibaraki, 305 Japan
7 Osaka Prefectural Agricultural and Forestry Research Center Habikino, Osaka, 583 Japan
8 Aomori Green BioCenter Aomori, 030-01 Japan
9To whom correspondence should be addressed.
A series of chimeric promoters for higher-level expression of foreign genes in plants was constructed as fusions of a gene for rß-glucuronidase (GUS) with the terminator of a gene for nopaline synthase (nos) or of the cauliflower mosaic virus (CaMV) 35S transcript, and the strength of these promoters was assayed in transient and stable expression systems in tobacco and rice. As parts of these promoters, the CaMV 35S core promoter, three different 5'-upstream sequences of the 35S promoter, the first intron of a gene for phaseolin, and a 5'-untranslated sequence (
sequence) of tobacco mosaic virus were used in various combinations. In tobacco and rice protoplasts, all three fragments of the 35S promoter (–419 to –90, –390 to –90 and –290 to –90, relative to the site of initiation of transcription), the intron, and the sequence effectively enhanced GUS activity. Some chimeric promoters allowed levels of GUS activity that were 20- to 70-fold higher than those obtained with the 35S promoter in pBI221. In tobacco protoplasts, the two longer fragments of the 35S promoter were more effective than the shortest fragment. In rice cells, by contrast, the shortest fragment was as effective as the two longer ones. The terminator of the 35S transcript was more effective than that of the nos gene for gene expression. In transgenic tobacco plants, a representative powerful promoter, as compared to the 35S promoter, allowed 10- and 50-fold higher levels of expression on average and at most, respectively, with no clear qualitative differences in tissue- and organ-specific patterns of expression. When the representative promoter was introduced into tobacco with a gene for luciferase, the autofluorescence of detached leaves after a supply of luciferin to petioles was great and was easily detectable by the naked eye in a dark room.
(Received June 9, 1995; Accepted October 30, 1995)
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Abe, Y. Hiwatashi, M. Ito, M. Hasebe, and H. Sekimoto Expression of Exogenous Genes Under the Control of Endogenous HSP70 and CAB Promoters in the Closterium peracerosum-strigosum-littorale complex Plant Cell Physiol., April 1, 2008; 49(4): 625 - 632. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Nagae, M. Nakata, and Y. Takahashi Identification of Negative cis-Acting Elements in Response to Copper in the Chloroplastic Iron Superoxide Dismutase Gene of the Moss Barbula unguiculata Plant Physiology, April 1, 2008; 146(4): 1687 - 1696. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sun, J.-P. Yu, F. Chen, T.-J. Zhao, X.-H. Fang, Y.-Q. Li, and S.-F. Sui TINY, a Dehydration-responsive Element (DRE)-binding Protein-like Transcription Factor Connecting the DRE- and Ethylene-responsive Element-mediated Signaling Pathways in Arabidopsis J. Biol. Chem., March 7, 2008; 283(10): 6261 - 6271. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kawakami, Y. Sato, and M. Yoshida Genetic engineering of rice capable of synthesizing fructans and enhancing chilling tolerance J. Exp. Bot., March 3, 2008; (2008) erm367v1. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Katsumoto, M. Fukuchi-Mizutani, Y. Fukui, F. Brugliera, T. A. Holton, M. Karan, N. Nakamura, K. Yonekura-Sakakibara, J. Togami, A. Pigeaire, et al. Engineering of the Rose Flavonoid Biosynthetic Pathway Successfully Generated Blue-Hued Flowers Accumulating Delphinidin Plant Cell Physiol., November 1, 2007; 48(11): 1589 - 1600. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ito, N. Nagata, Y. Yoshiba, M. Ohme-Takagi, H. Ma, and K. Shinozaki Arabidopsis MALE STERILITY1 Encodes a PHD-Type Transcription Factor and Regulates Pollen and Tapetum Development PLANT CELL, November 1, 2007; 19(11): 3549 - 3562. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hibi, S. Kosugi, T. Iwai, M. Kawata, S. Seo, I. Mitsuhara, and Y. Ohashi Involvement of EIN3 homologues in basic PR gene expression and flower development in tobacco plants J. Exp. Bot., October 26, 2007; (2007) erm216v1. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Ogawa, K. Yamaguchi, and T. Nishiuchi High-level overexpression of the Arabidopsis HsfA2 gene confers not only increased themotolerance but also salt/osmotic stress tolerance and enhanced callus growth J. Exp. Bot., September 20, 2007; (2007) erm184v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Hara, R. Kajita, K. U. Torii, D. C. Bergmann, and T. Kakimoto The secretory peptide gene EPF1 enforces the stomatal one-cell-spacing rule Genes & Dev., July 15, 2007; 21(14): 1720 - 1725. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Goto, T. Kobori, Y. Kosaka, T. Natsuaki, and C. Masuta Characterization of Silencing Suppressor 2b of Cucumber Mosaic Virus Based on Examination of its Small RNA-Binding Abilities Plant Cell Physiol., July 1, 2007; 48(7): 1050 - 1060. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Takabatake, E. Karita, S. Seo, I. Mitsuhara, K. Kuchitsu, and Y. Ohashi Pathogen-Induced Calmodulin Isoforms in Basal Resistance Against Bacterial and Fungal Pathogens in Tobacco Plant Cell Physiol., March 1, 2007; 48(3): 414 - 423. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Takabatake, Y. Ando, S. Seo, S. Katou, S. Tsuda, Y. Ohashi, and I. Mitsuhara MAP Kinases Function Downstream of HSP90 and Upstream of Mitochondria in TMV Resistance Gene N-Mediated Hypersensitive Cell Death Plant Cell Physiol., March 1, 2007; 48(3): 498 - 510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Inui, K.-i. Tamura, N. Fujii, T. Morishige, and F. Sato Overexpression of Coptis japonica Norcoclaurine 6-O-Methyltransferase Overcomes the Rate-Limiting Step in Benzylisoquinoline Alkaloid Biosynthesis in Cultured Eschscholzia californica Plant Cell Physiol., February 1, 2007; 48(2): 252 - 262. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yaegashi, T. Takahashi, M. Isogai, T. Kobori, S. Ohki, and N. Yoshikawa Apple chlorotic leaf spot virus 50 kDa movement protein acts as a suppressor of systemic silencing without interfering with local silencing in Nicotiana benthamiana J. Gen. Virol., January 1, 2007; 88(1): 316 - 324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Suzuki, S. Takahashi, R. Watanabe, Y. Fukushima, N. Fujita, A. Noguchi, R. Yokoyama, K. Nishitani, T. Nishino, and T. Nakayama An Isoflavone Conjugate-hydrolyzing beta-Glucosidase from the Roots of Soybean (Glycine max) Seedlings: PURIFICATION, GENE CLONING, PHYLOGENETICS, AND CELLULAR LOCALIZATION J. Biol. Chem., October 6, 2006; 281(40): 30251 - 30259. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Ono, M. Fukuchi-Mizutani, N. Nakamura, Y. Fukui, K. Yonekura-Sakakibara, M. Yamaguchi, T. Nakayama, T. Tanaka, T. Kusumi, and Y. Tanaka Yellow flowers generated by expression of the aurone biosynthetic pathway PNAS, July 18, 2006; 103(29): 11075 - 11080. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Sakuma, K. Maruyama, Y. Osakabe, F. Qin, M. Seki, K. Shinozaki, and K. Yamaguchi-Shinozaki Functional Analysis of an Arabidopsis Transcription Factor, DREB2A, Involved in Drought-Responsive Gene Expression PLANT CELL, May 1, 2006; 18(5): 1292 - 1309. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Yoshida, T. Umezawa, T. Mizoguchi, S. Takahashi, F. Takahashi, and K. Shinozaki The Regulatory Domain of SRK2E/OST1/SnRK2.6 Interacts with ABI1 and Integrates Abscisic Acid (ABA) and Osmotic Stress Signals Controlling Stomatal Closure in Arabidopsis J. Biol. Chem., February 24, 2006; 281(8): 5310 - 5318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Furihata, K. Maruyama, Y. Fujita, T. Umezawa, R. Yoshida, K. Shinozaki, and K. Yamaguchi-Shinozaki Abscisic acid-dependent multisite phosphorylation regulates the activity of a transcription activator AREB1 PNAS, February 7, 2006; 103(6): 1988 - 1993. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Takabatake, S. Seo, I. Mitsuhara, S. Tsuda, and Y. Ohashi Accumulation of the Two Transcripts of the N gene, Conferring Resistance to Tobacco Mosaic Virus, is Probably Important for N Gene-dependent Hypersensitive Cell Death Plant Cell Physiol., February 1, 2006; 47(2): 254 - 261. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Radi, T. Lange, T. Niki, M. Koshioka, and M. J. P. Lange Ectopic Expression of Pumpkin Gibberellin Oxidases Alters Gibberellin Biosynthesis and Development of Transgenic Arabidopsis Plants Plant Physiology, February 1, 2006; 140(2): 528 - 536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kajikawa, K. T. Yamato, Y. Kohzu, S.-i. Shoji, K. Matsui, Y. Tanaka, Y. Sakai, and H. Fukuzawa A Front-end Desaturase from Chlamydomonas reinhardtii Produces Pinolenic and Coniferonic Acids by {omega}13 Desaturation in Methylotrophic Yeast and Tobacco Plant Cell Physiol., January 1, 2006; 47(1): 64 - 73. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Gomi, D. Ogawa, S. Katou, H. Kamada, N. Nakajima, H. Saji, T. Soyano, M. Sasabe, Y. Machida, I. Mitsuhara, et al. A Mitogen-activated Protein Kinase NtMPK4 Activated by SIPKK is Required for Jasmonic Acid Signaling and Involved in Ozone Tolerance via Stomatal Movement in Tobacco Plant Cell Physiol., December 1, 2005; 46(12): 1902 - 1914. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ifuku, Y. Yamamoto, T.-a. Ono, S. Ishihara, and F. Sato PsbP Protein, But Not PsbQ Protein, Is Essential for the Regulation and Stabilization of Photosystem II in Higher Plants Plant Physiology, November 1, 2005; 139(3): 1175 - 1184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kabeya and N. Sato Unique Translation Initiation at the Second AUG Codon Determines Mitochondrial Localization of the Phage-Type RNA Polymerases in the Moss Physcomitrella patens Plant Physiology, May 1, 2005; 138(1): 369 - 382. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Matarasso, S. Schuster, and A. Avni A Novel Plant Cysteine Protease Has a Dual Function as a Regulator of 1-Aminocyclopropane-1-Carboxylic Acid Synthase Gene Expression PLANT CELL, April 1, 2005; 17(4): 1205 - 1216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Osakabe, K. Maruyama, M. Seki, M. Satou, K. Shinozaki, and K. Yamaguchi-Shinozaki Leucine-Rich Repeat Receptor-Like Kinase1 Is a Key Membrane-Bound Regulator of Abscisic Acid Early Signaling in Arabidopsis PLANT CELL, April 1, 2005; 17(4): 1105 - 1119. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Umezawa, R. Yoshida, K. Maruyama, K. Yamaguchi-Shinozaki, and K. Shinozaki SRK2C, a SNF1-related protein kinase 2, improves drought tolerance by controlling stress-responsive gene expression in Arabidopsis thaliana PNAS, December 7, 2004; 101(49): 17306 - 17311. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Schaarschmidt, N. Qu, D. Strack, U. Sonnewald, and B. Hause Local Induction of the alc Gene Switch in Transgenic Tobacco Plants by Acetaldehyde Plant Cell Physiol., November 15, 2004; 45(11): 1566 - 1577. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yasuhara, S. Mitsui, H. Hirano, R. Takanabe, Y. Tokioka, N. Ihara, A. Komatsu, M. Seki, K. Shinozaki, and T. Kiyosue Identification of ASK and clock-associated proteins as molecular partners of LKP2 (LOV kelch protein 2) in Arabidopsis J. Exp. Bot., September 1, 2004; 55(405): 2015 - 2027. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Qin, Y. Sakuma, J. Li, Q. Liu, Y.-Q. Li, K. Shinozaki, and K. Yamaguchi-Shinozaki Cloning and Functional Analysis of a Novel DREB1/CBF Transcription Factor Involved in Cold-Responsive Gene Expression in Zea mays L. Plant Cell Physiol., August 15, 2004; 45(8): 1042 - 1052. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kasahara, T. Kagawa, Y. Sato, T. Kiyosue, and M. Wada Phototropins Mediate Blue and Red Light-Induced Chloroplast Movements in Physcomitrella patens Plant Physiology, July 1, 2004; 135(3): 1388 - 1397. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kasuga, S. Miura, K. Shinozaki, and K. Yamaguchi-Shinozaki A Combination of the Arabidopsis DREB1A Gene and Stress-Inducible rd29A Promoter Improved Drought- and Low-Temperature Stress Tolerance in Tobacco by Gene Transfer Plant Cell Physiol., March 15, 2004; 45(3): 346 - 350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Konishi, Y. Ohmiya, and T. Hayashi Evidence That Sucrose Loaded into the Phloem of a Poplar Leaf Is Used Directly by Sucrose Synthase Associated with Various {beta}-Glucan Synthases in the Stem Plant Physiology, March 1, 2004; 134(3): 1146 - 1152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Aoki, S. Kato, K. Ichikawa, and M. Shimizu Circadian Expression of the PpLhcb2 Gene Encoding a Major Light-Harvesting Chlorophyll a/b-Binding Protein in the Moss Physcomitrella patens Plant Cell Physiol., January 15, 2004; 45(1): 68 - 76. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fukuchi-Mizutani, H. Okuhara, Y. Fukui, M. Nakao, Y. Katsumoto, K. Yonekura-Sakakibara, T. Kusumi, T. Hase, and Y. Tanaka Biochemical and Molecular Characterization of a Novel UDP-Glucose:Anthocyanin 3'-O-Glucosyltransferase, a Key Enzyme for Blue Anthocyanin Biosynthesis, from Gentian Plant Physiology, July 1, 2003; 132(3): 1652 - 1663. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Yoshida, T. Hobo, K. Ichimura, T. Mizoguchi, F. Takahashi, J. Aronso, J. R. Ecker, and K. Shinozaki ABA-Activated SnRK2 Protein Kinase is Required for Dehydration Stress Signaling in Arabidopsis Plant Cell Physiol., December 15, 2002; 43(12): 1473 - 1483. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Suzuki, T. Nakayama, K. Yonekura-Sakakibara, Y. Fukui, N. Nakamura, M.-a. Yamaguchi, Y. Tanaka, T. Kusumi, and T. Nishino cDNA Cloning, Heterologous Expressions, and Functional Characterization of Malonyl-Coenzyme A:Anthocyanidin 3-O-Glucoside-6"-O-Malonyltransferase from Dahlia Flowers Plant Physiology, December 1, 2002; 130(4): 2142 - 2151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.-H. Jeong, S. An, H.-G. Kang, S. Moon, J.-J. Han, S. Park, H. S. Lee, K. An, and G. An T-DNA Insertional Mutagenesis for Activation Tagging in Rice Plant Physiology, December 1, 2002; 130(4): 1636 - 1644. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Imaizumi, A. Kadota, M. Hasebe, and M. Wada Cryptochrome Light Signals Control Development to Suppress Auxin Sensitivity in the Moss Physcomitrella patens PLANT CELL, February 1, 2002; 14(2): 373 - 386. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Mitsuhara, N. Shirasawa-Seo, T. Iwai, S. Nakamura, R. Honkura, and Y. Ohashi Release From Post-transcriptional Gene Silencing by Cell Proliferation in Transgenic Tobacco Plants: Possible Mechanism for Noninheritance of the Silencing Genetics, January 1, 2002; 160(1): 343 - 352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. F. Schultz, T. Kiyosue, M. Yanovsky, M. Wada, and S. A. Kay A Role for LKP2 in the Circadian Clock of Arabidopsis PLANT CELL, December 1, 2001; 13(12): 2659 - 2670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Fukayama, H. Tsuchida, S. Agarie, M. Nomura, H. Onodera, K. Ono, B.-H. Lee, S. Hirose, S. Toki, M. S.B. Ku, et al. Significant Accumulation of C4-Specific Pyruvate, Orthophosphate Dikinase in a C3 Plant, Rice Plant Physiology, November 1, 2001; 127(3): 1136 - 1146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ishimaru, T. Hirose, N. Aoki, S. Takahashi, K. Ono, S. Yamamoto, J. Wu, S. Saji, T. Baba, M. Ugaki, et al. Antisense Expression of a Rice Sucrose Transporter OsSUT1 in Rice (Oryza sativa L.) Plant Cell Physiol., October 1, 2001; 42(10): 1181 - 1185. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Niki, T. Nishijima, M. Nakayama, T. Hisamatsu, N. Oyama-Okubo, H. Yamazaki, P. Hedden, T. Lange, L. N. Mander, and M. Koshioka Production of Dwarf Lettuce by Overexpressing a Pumpkin Gibberellin 20-Oxidase Gene Plant Physiology, July 1, 2001; 126(3): 965 - 972. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kushnir, E. Babiychuk, S. Storozhenko, M. W. Davey, J. Papenbrock, R. De Rycke, G. Engler, U. W. Stephan, H. Lange, G. Kispal, et al. A Mutation of the Mitochondrial ABC Transporter Sta1 Leads to Dwarfism and Chlorosis in the Arabidopsis Mutant starik PLANT CELL, January 1, 2001; 13(1): 89 - 100. [Abstract] [Full Text]
|
|
|
|
|
|
H. Batoko, H.-Q. Zheng, C. Hawes, and I. Moore A Rab1 GTPase Is Required for Transport between the Endoplasmic Reticulum and Golgi Apparatus and for Normal Golgi Movement in Plants PLANT CELL, November 1, 2000; 12(11): 2201 - 2218. [Abstract] [Full Text]
|
|
|
|
|
|
H. Satoh, H. Matsuda, T. Kawamura, M. Isogai, N. Yoshikawa, and T. Takahashi Intracellular distribution, cell-to-cell trafficking and tubule-inducing activity of the 50 kDa movement protein of Apple chlorotic leaf spot virus fused to green fluorescent protein J. Gen. Virol., August 1, 2000; 81(8): 2085 - 2093. [Abstract] [Full Text]
|
|
|
|
|
|
S. Takahashi, K. Ono, M. Ugaki, K. Ishimaru, N. Aoki, and R. Ohsugi Ser162-Dependent Inactivation of Overproduced Sucrose-Phosphate Synthase Protein of Maize Leaf in Transgenic Rice Plants Plant Cell Physiol., July 1, 2000; 41(8): 977 - 981. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Aoki and K. Syono Horizontal gene transfer and mutation: Ngrol genes in the genome of Nicotiana glauca PNAS, November 9, 1999; 96(23): 13229 - 13234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hamada, H. Kodama, K. Takeshita, H. Utsumi, and K. Iba Characterization of Transgenic Tobacco with an Increased alpha -Linolenic Acid Level Plant Physiology, October 1, 1998; 118(2): 591 - 598. [Abstract] [Full Text]
|
|
|
|
|
|
Q. Liu, M. Kasuga, Y. Sakuma, H. Abe, S. Miura, K. Yamaguchi-Shinozaki, and K. Shinozaki Two Transcription Factors, DREB1 and DREB2, with an EREBP/AP2 DNA Binding Domain Separate Two Cellular Signal Transduction Pathways in Drought- and Low-Temperature-Responsive Gene Expression, Respectively, in Arabidopsis PLANT CELL, August 1, 1998; 10(8): 1391 - 1406. [Abstract] [Full Text]
|
|
|
|
|
|
F. Sato, T. Hashimoto, A. Hachiya, K.-i. Tamura, K.-B. Choi, T. Morishige, H. Fujimoto, and Y. Yamada Inaugural Article: Metabolic engineering of plant alkaloid biosynthesis PNAS, January 2, 2001; 98(1): 367 - 372. [Abstract] [Full Text] [PDF]
|
|