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Plant and Cell Physiology, 1969, Vol. 10, No. 2 307-315
© 1969

Article

Germination of Phaseolus vulgaris III. The role of nucleic acid and protein synthesis in the initiation of axis elongation1

D. C. WALTON and G. S. SOOFI

Department of Chemistry, State University College of Forestry Syracuse, N. Y., U. S. A.

Excised embryonic axes of Phaseolus vulgaris L. (var. White Marrowfat) begin cell elongation after approximately 4 hr of incubation at 26°C. The incorporation of 32P into nucleic acids and phenylalanine-l-14C into protein markedly increases during the 4th hr of incubation, prior to initiation of cell elongation.

CH, which inhibits incorporation of phenylalanine-l-14C into protein by 93% during the 2nd hr after its addition, completely prevents the initiation of axis elongation if added up to 2 hr after the beginning of imbibition. Actinomycin D reduces the fresh weight increase of the axes, and inhibits both 32P incorporation into nucleic acids and phenylalanine-l-14C incorporation into protein. 5-FU inhibits 32P incorporation into nucleic acids but not phenylalanine-l-14C incorporation into protein or the fresh weight increase of the axes.

MAK column chromatography indicates that actinomycin D inhibits the synthesis of all types of nucleic acids to about the same extent, while 5-FU almost completely inhibits the accumulation of 32P in ribosomal RNA with lesser but significant inhibitory effects on accumulation of 32P in tRNA.

The results suggest an absolute requirement for protein synthesis prior to initiation of cell elongation and at least a partial requirement for synthesis of nucleic acid species other than ribosomal RNA, tRNA and DNA. The kinetic data suggest that the axes develop a greatly increased capacity for nucleic acid and protein synthesis prior to initiation of axis elongation.

1This research was supported by NSF grant GB 4145 and a grant from the U. S. Forest Service.

(Received December 16, 1968; )
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