Divergence of Evolutionary Ways Among Common sym Genes: CASTOR and CCaMK Show Functional Conservation Between Two Symbiosis Systems and Constitute the Root of a Common Signaling Pathway
Plant Cell Physiol. Banba et al.
49: 1659
Supplementary Data
files
Files in this Data Supplement:
- Supplementary Data 1
-
Supplemental Figure 1. Symbiotic phenotypes of the vector control roots of the Ljccamk, Ljcastor, and Ljpollux
Symbiotic phenotypes of hairy roots transformed with an empty vector (containing marker only) are shown as the controls for Figures 3, 4, 5 and 6. Experimental conditions are exactly the same as indicated in the legends for those Figures. Arrowheads indicate aberrant appressoria or hyphal swelling. Scale bars: A, C, and E, 0.1 mm; B, D, and F, 0.02 mm; G-L, 2 mm.
(A and B) Trypan blue-stained Ljccamk roots transformed with an empty vector lacking a CCaMK expression cassette. No mycorrhizal colonization was observed inside the roots. Hyphal swellings and aberrant appressorium indicate AM infections are blocked at the epidermis.
(C and D) Trypan blue-stained Ljcastor roots transformed with an empty vector lacking a CASTOR expression cassette showed the AM defective phenotype.
(E and F) Trypan blue-stained Ljpollux roots transformed with an empty vector lacking a POLLUX expression cassette showed the AM defective phenotype.
(G and H) No nodule was formed after inoculation with M. loti in Ljccamk roots transformed with an empty vector.
(I and J) No nodule was formed after inoculation with M. loti in Ljcastor roots transformed with an empty vector.
(K and L) No nodule was formed after inoculation with M. loti in Ljpollux roots transformed with an empty vector.
- Supplementary Data 2
-
Supplemental Figure 2. Symbiotic phenotypes of the Ljpollux roots transformed with PLjPOL:OsPOLLUX
Symbiotic phenotypes of the Ljpollux roots transformed with OsPOLLUX under the control of the LjPollux native promoter (PLjPOL). Experimental conditions are the same as in Figure 7. Scale bars: A and B, 0.1 mm; C-F, 2 mm.
(A and B) Trypan blue-stained Ljpollux roots transformed with PLjPOL:OsPOLLUX. AM infection was blocked at root epidermis. Arrowheads indicate aberrant appressoria. rh, ramified hyphae.
(C and D) No nodule was formed after inoculation with M. loti in Ljpollux harboring PLjPOL:OsPOLLUX.
(E and F) In rare occasions, small bumps were formed on Ljpollux roots transformed with PLjPOL:OsPOLLUX.
- Supplementary Data 3
-
Supplemental Table 1. doc file
