Skip Navigation



Plant and Cell Physiology Advance Access published online on November 2, 2009
Plant and Cell Physiology, doi:10.1093/pcp/pcp160
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow Supplementary Data
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Ueno, D.
Right arrow Articles by Ma, J. F.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ueno, D.
Right arrow Articles by Ma, J. F.
Agricola
Right arrow Articles by Ueno, D.
Right arrow Articles by Ma, J. F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Identification of a novel major quantitative trait locus controlling distribution of Cd between roots and shoots in rice

Daisei Ueno1, Emi Koyama1, Izumi Kono2, Tsuyu Ando2, Masahiro Yano3 and Jian Feng Ma1,*

1 Research Institute for Bioresources, Okayama University, Chuo 2-20-1, Kurashiki 710-0046, Japan
2 Institute of Society for Techno-innovation of Agriculture, Forestry, and Fisheries, Kamiyokoba, Tsukuba, Ibaraki, 305-0854, Japan
3 National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan

* Corresponding author: Jian Feng Ma Research Institute for Bioresources, Okayama University, Chuo 2-20-1, Kurashiki 710-0046, Japan Tel/Fax: +81-86-434-1209 E-mail: maj{at}rib.okayama-u.ac.jp


  Abstract

Accumulation of Cd in rice grain is a serious concern of food safety since rice as a staple food is a major source of Cd intake in Asian countries. However, the mechanisms controlling Cd accumulation in rice are still poorly understood. Herein, we performed both physiological and genetic analysis of two rice cultivars contrasting in Cd accumulation, which were screened from a core-collection of rice cultivars. The cultivar Anjana Dhan (Indica) accumulated much higher Cd than Nipponbare (Japonica) in the shoots and grains when grown in both soil and solution culture. A short-term uptake experiment (20 min) showed that Cd uptake by Nipponbare was higher than that by Anjana Dhan. However, the concentration of Cd in the shoot and xylem sap was much higher in Anjana Dhan than in Nipponbare. Of the Cd taken up by the roots, less than 4% was translocated to the shoots in Nipponbare, compared with 10-25% in Anjana Dhan, indicating a higher root-to-shoot translocation of Cd in the latter. A quantitative trait locus (QTL) analysis for Cd accumulation was performed using a F2 population derived from Anjana Dhan and Nipponbare. A QTL with large effect for Cd accumulation was detected on the short arm of chromosome 7, explaining 85.6% of the phenotypic variance in the shoot Cd concentration of the F2 population. High accumulation is likely to be controlled by a single recessive gene. Candidate genomic region was defined less than 1.9 Mb by mean of substitution mapping.

Keywords: Rice (Oryza sative) - Cd accumulation - QTL - uptake - root-to-shoot translocation

(Received October 2, 2009; Accepted October 28, 2009)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.