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Plant and Cell Physiology Advance Access published online on November 3, 2009
Plant and Cell Physiology, doi:10.1093/pcp/pcp159
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Arabidopsis NAC transcription factor, ANAC078, regulates flavonoids biosynthesis under high-light

Teruyuki Morishita1, Yusuke Kojima1, Takanori Maruta1, Ayako Nishizawa-Yokoi1, Yukinori Yabuta2 and Shigeru Shigeoka1,{dagger}

1Department of Advanced Bioscience, Faculty of Agriculture, Kinki University, 3327-204 Nakamachi, Nara 631-8505, Japan
2School of Agricultural, Biological, and Environmental Sciences, Faculty of Agriculture, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, Japan

Corresponding Author: Shigeru Shigeoka Department of Advanced Bioscience, Faculty of Agriculture, Kinki University, 3327-204 Nakamachi, Nara 631-8505, Japan Tel: +81-742-43-8083 Fax: +81-742-43-8976 e-mail: shigeoka{at}nara.kindai.ac.jp


  Abstract

We have isolated a combination of high-light and heat-shock (HL + HS) stress-inducible genes, including a NAC transcription factor designated ANAC078 (Nishizawa et al. 2006). Here we explored the physiological function of ANAC078 under HL stress. Yeast transcription activity assays showed that ANAC078 functions as a transcriptional activator. A fusion protein composed of green fluorescent protein (GFP) and the full-length ANAC078 was detected in the nucleus and cytoplasm, while fusion proteins comprising GFP and ANAC078 deleted of a putative transmembrane motif were found only in the nucleus. In ANAC078-overexpressing Arabidopsis plants (Ox-ANAC078-43), the transcription of 166 genes was up-regulated compared with the levels in wild-type plants under HL (1,200 µmol m-2 s-1, 30°C). These genes included some for transcription factors regulating the expression of genes related to the biosynthesis of flavonoids. Interestingly, the transcript levels of some genes related to flavonoid biosynthesis and the levels of anthocyanins were significantly increased in the Ox-ANAC0478 plants and reduced in knock-out ANAC078 plants (KO-ANAC078) compared with the wild-type plants under HL stress.

The present findings suggest that ANAC078 protein is associated with the induction of genes related to flavonoid biosynthesis, leading to the accumulation of anthocyanins, in response to HL stress.

Keywords: Arabidopsis - flavonoid - high-light stress - NAC - transcription factor

(Received September 14, 2009; Accepted October 28, 2009)
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