Skip Navigation



Plant and Cell Physiology Advance Access published online on November 2, 2009
Plant and Cell Physiology, doi:10.1093/pcp/pcp153
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Ozawa, R.
Right arrow Articles by Takabayashi, J.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ozawa, R.
Right arrow Articles by Takabayashi, J.
Agricola
Right arrow Articles by Ozawa, R.
Right arrow Articles by Takabayashi, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Exogenous Polyamines Elicit Herbivore-Induced Volatiles in Lima Bean Leaves: Involvement of Calcium, H2O2 and Jasmonic Acid

Rika Ozawa1,*, Cinzia M. Bertea2,*, Maria Foti2, Ravishankar Narayana2, Gen-Ichiro Arimura1,3, Atsushi Muroi1, Jun-Ichiro Horiuchi4, Takaaki Nishioka5,**, Massimo E. Maffei2 and Junji Takabayashi1

1 Center for Ecological Research, Kyoto University, Otsu, 520-2113, Japan and Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation, Kawaguchi, 332-0012, Japan
2 Plant Physiology Unit, Dept. Plant Biology and Centre of Excellence CEBIOVEM, University of Turin, Via Quarello 15, 10135 Turin, Italy.
3 Global COE Program: Evolution and Biodiversity, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan
4 Laboratory of Insect Physiology, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan
5 Laboratory of Biofunction Chemistry, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan (T.N.)

Corresponding Author: Prof. Junji Takabayashi Center for Ecological Research, Kyoto University, Otsu, 520-2113, Japan Tel/Fax +81-77-549-8235 Email: junji{at}ecology.kyoto-u.ac.jp


  Abstract

We investigated the role of polyamines (PAs) in lima bean (Phaseolus lunatus) leaves on the production of herbivorous mite (Tetranuchus urticae)-induced plant volatiles that attract carnivorous natural enemies of the herbivores. To do this, we focused on the effects of the exogenous PAs [cadaverine, putrescine, spermidine and spermine (Spm)] on the production of volatiles, H2O2 and jasmonic acid (JA) and the levels of defensive genes, cytosolic calcium and reactive oxygen species (ROS). Among the tested PAs, Spm was most active in inducing the production of volatile terpenoids known to be induced by T. urticae. An increase in JA levels was also found after Spm treatment, indicating that Spm induces the biosynthesis of JA, which has been shown elsewhere to regulate the production of some volatile terpenoids. Further, treatment with JA and Spm together resulted in greater volatile emission than that with JA alone. In a Y-tube olfactometer, leaves treated with Spm+JA attracted more predatory mites (Phytoseiulus persimilis) than those treated with JA alone. After treatment with Spm+JA, no effects were found on enzyme activity of polyamine oxidase (PAO) and Cu-amine oxidase (CuAO). However, induction of calcium influx and ROS production, and increased enzyme activities and gene expressions for NADPH-oxidase complex, superoxide dismutase, catalase, ascorbate peroxidase, glutathione reductase and glutathione peroxidase, were found after treatment with Spm+JA. These results indicate that Spm plays an important role in the production of T. urticae-induced lima bean leaf volatiles.

Keywords: Polyamines - Lima bean - Herbivore induced volatile organic compounds - Calcium and ROS signaling - Jasmonic acid - Quantitative gene expression

* These authors contributed equally.

* * Present address: Institute for Advanced Biosciences 403-1 Daihoji, Tsuruoka, Yamagata 997-0017 Japan

(Received September 7, 2009; Accepted October 22, 2009)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.