Skip Navigation



Plant and Cell Physiology Advance Access published online on October 29, 2009
Plant and Cell Physiology, doi:10.1093/pcp/pcp150
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow Supplementary Data
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kwon, S. H.
Right arrow Articles by Kim, J. H.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kwon, S. H.
Right arrow Articles by Kim, J. H.
Agricola
Right arrow Articles by Kwon, S. H.
Right arrow Articles by Kim, J. H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Overexpression of a Brassica rapa NGATHA Gene in Arabidopsis thaliana Negatively Affects Cell Proliferation during Lateral Organ and Root Growth.

So Hyun Kwon1, Byung Ha Lee1, Eun Yu Kim2, Young Sam Seo2, Sangman Lee3, Woo Taek Kim2, Jong Tae Song3,* and Jeong Hoe Kim1,*

1Department of Biology, Kyungpook National University, Daegu 702-701, Korea
2Department of Biology, Yonsei University, Seoul 120-749, Korea
3School of Applied Bioscience, Kyungpook National University, Daegu 702-701, Korea

Corresponding authors: Jeong Hoe Kim Department of Biology Kyungpook National University Daegu 702-701, Korea Phone: +82-53-950-5347 Fax: +82-53-953-3066 E-mail: kimjeon4{at}knu.ac.kr Jong Tae Song School of Applied Biosciences Kyungpook National University Daegu 702-701, Korea Phone: +82-53-950-7753 Fax: +82-53-958-6880 E-mail: jtsong68{at}knu.ac.kr


  Abstract

In an effort to elucidate biological functions of transcription factors of Brassica rapa L. (ssp. pekinensis), an NGATHA homologue, BrNGA1, that belongs to the B3-type transcription factor superfamily was identified and expressed in Arabidopsis thaliana under the control of the CaMV 35S promoter. Arabidopsis plants overexpressing BrNGA1, named BrNGA1ox, displayed markedly reduced organ growth compared to the wild type: lateral organs, such as leaves, flowers, and cotyledons, were small and distinctively narrow, and their root growth was severely retarded as well. Reduced sizes of BrNGA1ox organs were mainly due to reduction in cell numbers. Kinematic analysis on leaf growth revealed that both the rate and duration of cell proliferation declined during organogenesis, which was consistent with the reduced expression of cyclin genes. Reduction in organ growth was strongly correlated with the small size of meristematic cell pools in the shoot and root meristems. Taken together, these data indicate that BrNGA1 acts as a negative regulator of cell proliferation and may do so, in part, by regulating the size of the meristematic cell pool.

Keywords: organ size and shape - Brassica rapa - Arabidopsis - NGATHA - B3 transcription factors

(Received September 28, 2009; Accepted October 19, 2009)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.