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Plant and Cell Physiology Advance Access originally published online on June 10, 2009
Plant and Cell Physiology 2009 50(7):1364-1381; doi:10.1093/pcp/pcp084
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

This article appears in the following Plant and Cell Physiology issue: Special Issue Articles: Omics and Bioinformatics [View the issue table of contents]

Transcript Profiling During Fiber Development Identifies Pathways in Secondary Metabolism and Cell Wall Structure That May Contribute to Cotton Fiber Quality

Yves Al-Ghazi1, Stéphane Bourot2, Tony Arioli2, Elizabeth S. Dennis1 and Danny J. Llewellyn1,*

1CSIRO Plant Industry, PO Box 1600, Canberra, ACT 2601, Australia
2Bayer CropScience, Technologiepark 38, 9052 Gent, Belgium

*Corresponding author: E-mail, Danny.Llewellyn{at}csiro.au; Fax, +61-2-6246500.


  Abstract

A global gene expression profiling study at different stages of fiber development was undertaken on two cotton species cultivated for fiber, Gossypium hirsutum (L.) and G. barbadense (L.). A large proportion of the genome was expressed during both fiber elongation and subsequent secondary cell wall thickening. There was a major shift in abundance of transcripts for gene regulation, cell organization and metabolism between fiber elongation and fiber thickening that was fundamentally similar in both species. Each stage had its own distinctive features represented by specific metabolic and regulatory genes, a number of which have been noted previously. Many of the genes expressed in the fibers were of a similar type and developmental expression to those seen in other fiber-producing plants, indicating a conservation of mechanisms of cell elongation and wall thickening across diverse plant genera. Secondary metabolism and pectin synthesis and modification genes were amongst the most statistically significant differentially expressed categories between the two species during fiber elongation. The gene profiles of the fiber thickening stage, however, were almost identical between the two species, suggesting that their different final fiber quality properties may be established at earlier stages of fiber development. Expression levels of repre-sentative phenylpropanoid and pectin modification genes showed high correlations with specific fiber properties in an inter-specific cotton recombinant inbred line (RIL) population, supporting a role in determining fiber quality.

Keywords: Cotton - Expression profiling - Fiber development - Fiber quality - Gossypium barbadense - Gossypium hirsutum - Microarray

Abbreviations: AGP, arabinogalactan protein; bHLH, basic helix–loop–helix protein; C4H, cinnamate 4-hydroxylase; CHS, chalcone synthase; 4CL, 4-hydroxycinnamoyl-CoA ligase; dpa, day post-anthesis; EST, expressed sequence tag; HQT, hydroxycinnamoyl-CoA:quinate hydroxycinnamoyl transferase; HVI, high volume instrumentation; ICE, inducer of CBF expression; IFR, isoflavone reductase; NAC, NAM/ATAF/CUC transcription factor; NAM, no apical meristem transcription factor; PAL, phenylalanine ammonia lyase; PCW, primary cell wall; PME. pectin methylesterase; Q-PCR, quantitative PCR; QTL, quantitiative trait locus; RIL, recombinant inbred line; RT–PCR, reverse transcription–PCR; SAM, S-adenosyl-L-methionine; SCW, secondary cell wall; TC, tentative consensus; TFU, total fiber units; XET, xyloglucan endotransglycosylase.

(Received March 2, 2009; Accepted June 5, 2009)
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