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Plant and Cell Physiology Advance Access originally published online on May 22, 2009
Plant and Cell Physiology 2009 50(7):1387-1392; doi:10.1093/pcp/pcp071
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

This article appears in the following Plant and Cell Physiology issue: Special Issue Articles: Omics and Bioinformatics [View the issue table of contents]

Short Communication

Differential Effects of a Transgene to Confer Low Phytic Acid in Caryopses Located at Different Positions in Rice Panicles

Mio Kuwano1, Fumio Takaiwa2 and Kaoru T. Yoshida1,*

1Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo. 113-8657 Japan
2National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki. 305-8602 Japan

*Corresponding author: E-mail, ayosida{at}mail.ecc.u-tokyo.ac.jp; Fax: +81-3-5841-1306.


  Abstract

In previous studies, we attempted to reduce phytic acid in rice seeds by silencing the 1D-myo-inositol 3-phosphate synthase gene, RINO1, using an antisense sequence under the control of the rice glutelin GluB-1 promoter. The stable transgenic line showed a weak low phytic acid phenotype. In this study, we show that the position of the caryopsis in the panicle might affect the level of gene silencing through a difference in temporal and spatial expression patterns between RINO1 and GluB-1 promoters, resulting in a large variation in Pi levels and a small increase in Pi in the transgenic seeds.

Keywords: Gene silencing - Glutelin promoter - Myo-inositol 3-phosphate synthase - Oryza sativa L. - Phytic acid - Transgenic plants

Abbreviations: DAF, days after flowering; GUS, β-glucuronidase; lpa, low phytic acid.

(Received April 7, 2009; Accepted May 19, 2009)
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