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Plant and Cell Physiology, 1967, Vol. 8, No. 3 439-445
© 1967

Article

HORMONAL REGULATION ON THE INDUCTION OF {alpha}-AMYLASE ISOZYMES IN THE EMBRYO-LESS ENDOSPERM OF BARLEY

YOSHIHIDE MOMOTANI and JIRO KATO

Department of Bioloty, University of Osaka Prefecture Sakai, Osaka

The {alpha}-amylase induced by helminthosporol and gibberellic acid in the embryo-less endosperm of barley was separated into the three fractions, {alpha}1, {alpha}2 and {alpha}3, by DEAE-cellulose column chromatography. A maximum amount of the {alpha}2. was induced by gibberellic acid and that of the as by helminthosporol. After rechromatography, the {alpha}2 induced by gibberellic acid and the as induced by helminthosporol showed their respective single bands in an electrophoresis agar gel zymogram. On the other hand, the ai induced by helminthosporol and gibberellic acid showed three bands.

Dihydrohelminthosporic acid, a derivative of helminthosporol, induced the same isozymes as helminthosporol did.

(Received May 8, 1967; )
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