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Plant and Cell Physiology, 1967, Vol. 8, No. 3 363-373
© 1967


Article

GROWTH OF CALLUS TISSUE FROM RICE-ROOT IN VIRTO

MICHIHIKO YATAZAWA, KATSUHISA FURUHASHI and MASAHARU SHIMIZU

Department of Agricultural Chemistry, Nagoya University Nagoya
Department of Agronomy, Faculty of Agriculture, Nagoya University Nagoya

The callus tissues which had been induced on the roots of rice seedlings by the action of 2,4-D were cultured on a modified HELLER medium for more than two years. The tissues were transferred into new msdium every two to three weeks. Yeast extract and 2,4-D were indispensable for maintaining the best growth. About fifty times' increase in tissue mass on a fresh weight basis was obtained after three weeks' incubation. Glucose, fructose, sucrose and maltose supported equally well the growth of callus tissue. The optimum temperature and pH were located between 25–30° and 5–6 respectively. Microscopic examination showed no sign of organ formation, but some tissue differentiation was observed at histological level.

(Received December 13, 1966; )
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