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Plant and Cell Physiology, 1967, Vol. 8, No. 2 249-262
© 1967

Article

CHANGE OF B-TYPE HAEM CONTENT IN RELATION TO PEROXIDASE BIOSYNTHESIS IN INJURED SWEET POTATO ROOTS1

YUKO KANAZAWA2, TADASHI ASAHI and IKUZO URITANI

Laboratory of Biochemistry, Faculty of Agriculture, Nagoya University Nagoya

The methods of quantitative analysis of b-type haem in plants were investigated. With an improved method developed was determined the haem content in the supernatant, mitochondrial, and microsomal fractions of sweet potato tissue. The activities of peroxidase, catalase, and cytochrome oxidase, as well as the contents of b-type haem and acid-insoluble nitrogen in the cellular fractions were determined at different incubation times after cutting of sweet potato tissue. Peroxidase and catalase increased with time in each celluler fraction, following a short lag phase. In the mitochondrial fraction, b-type haem, cytochrome oxidase, and acid insoluble nitrogen increased linearly with time. In the microsomal and supernatant fraction, b-type haem increased with time following a short lag phase. The increase in haem content of the supernatant fraction appeared to be associated with peroxidase formation.

Time course analysis showed that 59Fe-incorporation into b-type haem of the supernatant fraction increased with time and that incorporation was markedly inhibited by blasticidin S. The incorporation of 59Fe into mitochondrial haem did not increase with time and was not inhibited by blasticidin S. Blasticidin S inhibited 59Fe-incorporation into microsomal haem. Time course analysis of b-type haem content, 59Fe-incorporation into b-type haem, and peroxidase activity suggest that in the injured tissue haem is synthesized from low molecular weight compounds and is incorporated into peroxidase as the haem moiety.

1 This paper constitutes Part 57 of the Phytopathological Chemistry of Sweet Potato with Black Rot.

2 Present address: Institute for Plant Virus Research, Chiba.

(Received November 25, 1966; )
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