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Plant and Cell Physiology, 1965, Vol. 6, No. 4 721-741
© 1965

Article

METABOLISM OF GLUCOSE IN THE PROCESS OF "GLUCOSE-BLEACHING" OF CHLORELLA PROTOTHECOIDES

MITSUO MATSUKA and EIJI HASE

Institute of Applied Microbiology, University of Tokyo, and Tokugawa Institute for Biological Research Tokyo

  1. As previously demonstrated, normal cells of Chlorella protothecoides are bleached with degeneration of chloroplasts when they are incubated, under aerobic conditions—either in the light or in darkness—, in a glucose-containing medium without added nitrogen source ("glucose-bleaching"). It was found in the present study that under the atmosphere of N2, neither bleaching nor growth of algal cells occurs in the dark, while in the light a significant growth of cells takes place with formation of a certain amount of chlorophyll.
  2. Studies on the effects of various inhibitors (ammonium ion, DNP, CMU, {alpha}-hydroxysulphonates, arsenate, cyanide, azide, and antimycin A) under different conditions showed that oxidative phosphorylation is a necessary process for the occurrence of the glucosebleaching as well as the assimilation of glucose (cellular growth). Under light-anaerobic conditions in the presence of glucose, assimilation of glucose (cellular growth) takes place being supported by photophosphorylation, but no bleaching occurs.
  3. When the algal cells in the course of bleaching were transferred to the glucose-free mineral medium, the cell growth ceased immediately but the cell bleaching proceeded for several hours before its cessation. The respiratory activity, which was high in the glucose-containing medium, became lower on transferring the algal cells into the glucose-free medium. The lowered level of respiration was maintained, for more than 8 hr after the transfer of cells to the glucose-free medium.
  4. When the cells in the course of bleaching were placed under the atmosphere of N2, the cell bleaching ceased almost instantaneously.
  5. Based on these observations and other inhibition experiments, it was inferred that a certain intermediate(s) produced by the aerobic respiration of glucose is closely associated with the occurrence of cell bleaching, and that an O2-requiring step may be involved in the process of chlorophyll degradation.

(Received September 9, 1965; )
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