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Plant and Cell Physiology, 1965, Vol. 6, No. 4 689-697
© 1965

Article

EFFECT OF PLASTOCYANIN ON PHOTOPHOSPHORYLATION IN ISOLATED CHLOROPLASTS

REIKO FUJITA, HIDEHIRO SAKURAI, SAKAE KATOH and ATUSI TAKAMIYA

Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo Tokyo

The effect of addition of plastocyanin on photophosphorylation in isolated chloroplasts was studied in comparison with its effect on photoreduction. Catalytic amounts of added plastocyanin stimulated the HILL reaction with NADP as oxidant, but the photophosphorylation coupled to this reaction was not influenced by the addition of plastocyanin. The addition of NH4+ or the complete phosphorylating system did not affect the plastocyanin-induced increase in rate of the photoreduction. The rate of photoreduction of NADP, FMN, or indigo carmine in the presence of DPIP and ascorbate was much accelerated by added plastocyanin, but these reactions were not coupled to ATP formation.

As reported earlier, isolated chloroplasts can utilize plastocyanin as oxidant in the HILL reaction. This reaction was found to be accompanied by ATP formation, but the efficiency in this case was somewhat lower than in photophosphorylation coupled to the HILL reaction with the usual oxidants.

The possible mechanism of these reactions is described briefly and a scheme for reactions of plastocyanin in the electron transfer in chloroplasts is presented.

(Received July 20, 1965; )
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