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Plant and Cell Physiology, 1965, Vol. 6, No. 4 661-669
© 1965

Article

RIBONUCLEIC ACID-POLYPHOSPHATE FROM ALGAE: III. HYDROLYSIS STUDIES

DAVID L. CORRELL

Radiation Biology Laboratory, Smithsonian Institution Washington D.C. 20560, U.S.A.

RNA-polyphosphate was isolated from synchronous Chlorella cells. After each of a series of hydrolytic treatments, RNA-polyphosphate was chromatographically analyzed by means of a two-column ion-exchange system. Alkaline hydrolysates contained primarily ribonu-cleotides, pyrophosphate, and tripolyphosphate. Acid hydrolysates contained ribonucleotides, purine bases, ribonucleosides, orthophosphate, and an unknown, inorganic, phosphorus-containing compound (X-P). Treatments with pancreatic ribonuclease, spleen phosphodiesterase, and yeast polyphosphatase left large amounts of RNA-polyphosphate fragments. Treatment with venom phosphodiesterase yielded a high molecular weight inorganic polyphosphate fraction free from RNA. Such material was hydrolyzed to pyro- and tripolyphosphate by potassium hydroxide, to orthophosphate and an unknown compound X-P by perchloric acid, and to ortho-, pyro-, and tripolyphosphate by hydroxylamine under ester hydrolysis conditions. Synthetic inorganic polyphosphate was stable to potassium hydroxide and hydroxylamine under the same conditions and yielded only orthophosphate upon perchloric acid hydrolysis. Both natural and synthetic polyphosphates were hydrolyzed to low molecular weight fragments by yeast polyphosphatase. The evidence at present indicates that in Chlorella polyphosphate is not a simple phosphate anhydride chain.

(Received June 14, 1965; )
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