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Plant and Cell Physiology, 1965, Vol. 6, No. 4 615-629
© 1965


Article

EFFECT OF {alpha}-HYDROXYSULFONATES ON PHOTOCHEMICAL REACTIONS OF SPINACH CHLOROPLASTS AND PARTICIPATION OF GLYOXYLATE IN PHOTOPHOSPHORYLATION1

KOZI ASADA, SHUNJI KITOH2, RYUICHIRO DEURA and ZENZABURO KASAI

Research Institute for Food Science, Kyoto University Kyoto

  1. The effect of {alpha}-hydroxy sulfonates and sulfite, inhibitors of glycolate oxidase, on the photochemical reactions of spinach chloroplasts was studied. The photo reduction of ferricyanide and NADP was not affected by the poisons, whereas the photophosphorylation and 14CO2 fixation were inhibited.
  2. Glyoxylate was photoreduced by the chloroplasts in the presence of PPNR and glyoxylate reductase, and this reduction was accelerated by the addition of NADP. ATP formation accompanied with the reduction of glyoxylate by the illuminated chloroplasts was observed. It is supposed that glyoxylate oxidizes the photoreduced NADPH2 or PPNR and thus the photophosphorylation is stimulated.

1A part of this paper was presented at the annual meeting of Agricultural Society of Japan, in August, 1964.

2Present address: Radiation Center of Osaka Prefecture, Sakai, Osaka.


(Received May 10, 1965; )
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