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Plant and Cell Physiology Advance Access originally published online on July 24, 2009
Plant and Cell Physiology 2009 50(9):1715-1720; doi:10.1093/pcp/pcp109
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

Transcriptome Analyses Revealed Diverse Expression Changes in ago1 and hyl1 Arabidopsis Mutants

Yukio Kurihara1, Eli Kaminuma2,3, Akihiro Matsui1, Makiko Kawashima1, Maho Tanaka1, Taeko Morosawa1, Junko Ishida1, Yoshiki Mochizuki2, Kazuo Shinozaki4, Tetsuro Toyoda2 and Motoaki Seki1,5,*

1Plant Genomic Network Research Team, Plant Functional Genomics Research Group, RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045 Japan
2Bioinformatics and Systems Engineering Division, RIKEN Yokohama Institute, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045 Japan
3Gene-Product Informatics Laboratory, Center for Information Biology and DNA Data Bank of Japan, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka, 411-8540 Japan
4Gene Discovery Research Group, RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045 Japan
5Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, Yokohama, Kanagawa, 244-0813 Japan

*Corresponding author: E-mail, mseki{at}psc.riken.jp; Fax, +81-45-503-9586.


  Abstract

MicroRNAs (miRNAs) are 20–24 nucleotide endogenous regulatory molecules conserved in higher eukaryotes. In Arabidopsis, miRNAs are produced through step-wise cleavages of primary miRNA precursors (pri-miRNAs) by DICER-LIKE1 (DCL1). This cleavage step is also supported by a double-stranded RNA-binding protein, HYPONASTIC LEAVES1 (HYL1). In many cases, mature miRNA is predominantly incorporated into an endonuclease, ARGONAUTE1 (AGO1), which degrades miRNA-targeted mRNAs. Here, we examined and revealed whole genome transcriptomes in ago1-25 and hyl1-2 mutants using tiling arrays. The data in this paper are valuable for understanding the relationship between the miRNA pathway and its effect on transcriptomes.

Keywords: AGO1 - Arabidopsis - HYL1 - MicroRNA - Tiling array

Abbreviations: AGO1, ARGONAUTE1; DCL1, DICER-LIKE1; FDR, false discovery rate; HYL1, HYPONASTIC LEAVES1; miRNA, microRNA; pri-miRNA, primary miRNA precursor; qRT–PCR, quantitative reverse transcriptase–PCR; TAIR8, The Arabidopsis Information Resource 8.

(Received May 14, 2009; Accepted July 15, 2009)
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