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Plant and Cell Physiology Advance Access originally published online on July 15, 2009
Plant and Cell Physiology 2009 50(9):1710-1714; doi:10.1093/pcp/pcp103
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

Identification of cis-Localization Elements that Target Glutelin RNAs to a Specific Subdomain of the Cortical Endoplasmic Reticulum in Rice Endosperm Cells

Haruhiko Washida, Sachiyo Kaneko, Naoko Crofts, Aya Sugino, Changlin Wang and Thomas W Okita1,*

Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA

*Corresponding author: E-mail, okita{at}wsu.edu; Fax, +1-509-335-7643.


  Abstract

Rice glutelin RNAs are localized to the cisternal endoplasmic reticulum (ER) by a regulated RNA transport process requiring specific cis-localization elements. We set out to identify these glutelin sequences by their dominant character of being able to re-direct the normal protein body ER localization of a maize 10 kDa {delta}-zein RNA to the cisternal ER. In situ RNA localization analysis showed that the glutelin RNA contains multiple cis-localization elements; two located at the 5' and 3' ends of the coding sequences and a third located within the 3'-untranslated region. These three regions contain two conserved sequences, suggesting that these RNA recognition signals may be sequence based.

Keywords: Cis-localization element - Endoplasmic reticulum - Glutelin - In situ RT-PCR - Rice - RNA targeting

Abbreviations: ER, endoplasmic reticulum; PB-ER, protein body ER; PSV, protein storage vacuole; UTR, untranslated region; nos, nopaline synthase; in situ RT–PCR, in situ reverse transcriptase-mediated PCR.

(Received May 26, 2009; Accepted July 9, 2009)
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