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Plant and Cell Physiology Advance Access originally published online on July 16, 2009
Plant and Cell Physiology 2009 50(9):1636-1650; doi:10.1093/pcp/pcp107
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Effects of Tobacco Ethylene Receptor Mutations on Receptor Kinase Activity, Plant Growth and Stress Responses

Tao Chen1, Jun Liu1, Gang Lei, Yun-Feng Liu, Zhi-Gang Li, Jian-Jun Tao, Yu-Jun Hao, Yang-Rong Cao, Qing Lin, Wan-Ke Zhang, Biao Ma, Shou-Yi Chen and Jin-Song Zhang*

Plant Gene Expression Center, National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

*Corresponding author: E-mail, jszhang{at}genetics.ac.cn, Fax, +86-10-64873428.


  Abstract

Ethylene receptor is the first component of ethylene signaling that regulates plant growth, development and stress responses. Previously, we have demonstrated that tobacco subfamily 2 ethylene receptor NTHK1 had Ser/Thr kinase activity, and overexpression of NTHK1 caused large rosette, reduced ethylene sensitivity, and increased salt sensitivity in transgenic Arabidopsis plants. Here we found that N-box mutation in the NTHK1 kinase domain abolished the kinase activity and led to disruption of NTHK1 roles in conferring reduced ethylene sensitivity and salt sensitive response in transgenic Arabidopsis plants. However, N-box mutation had partial effects on NTHK1 regulation of rosette growth and expression of salt- and ethylene-responsive genes AtNAC2, AtERF1 and AtCor6.6. Mutation of conserved residues in the H box did not affect kinase activity, seedling growth, ethylene sensitivity or salt-induced epinasty in transgenic plants but did influence NTHK1 function in control of specific salt- and ethylene-responsive gene expression. Compared with NTHK1, the tobacco subfamily 1 ethylene receptor NtETR1 had His kinase activity and played a weak role in regulation of rosette growth, triple response and salt response. Mutation of the conserved His residue in the NtETR1 H box eliminated phosphorylation and altered the effect of Ntetr1-1 on reporter gene activity. These results imply that the Ser/Thr kinase activity of NTHK1 is differentially required for various responses, and NTHK1 plays a larger role than NtETR1.

Keywords: Ethylene receptor - Mutation - Ser/Thr kinase activity - Salt stress response - Signaling

Abbreviations: ACC, 1-aminocyclopropane-1-caboxylic acid; GST, glutathione S-transferase

1These authors contributed equally to the work.

(Received April 28, 2009; Accepted July 14, 2009)
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