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Plant and Cell Physiology Advance Access originally published online on June 3, 2009
Plant and Cell Physiology 2009 50(7):1393-1399; doi:10.1093/pcp/pcp080
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

This article appears in the following Plant and Cell Physiology issue: Special Issue Articles: Omics and Bioinformatics [View the issue table of contents]

Short Communication

Common Sets of Promoter Elements Determine the Expression Characteristics of Three Arabidopsis Genes Encoding Isoforms of Mitochondrial Cytochrome c Oxidase Subunit 6b

Eduardo F. Mufarrege, Graciela C. Curi and Daniel H. Gonzalez*

Instituto de Agrobiotecnología del Litoral (CONICET-UNL), Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, CC 242 Paraje El Pozo, 3000 Santa Fe, Argentina

*Corresponding author: E-mail, dhgonza{at}fbcb.unl.edu.ar; Fax: +54-342-4575219.


  Abstract

The promoters of the three Arabidopsis nuclear genes encoding mitochondrial cytochrome c oxidase subunit 6b (AtCOX6b) have similar expression patterns, with preferential expression in anthers and meristems, and are induced by sucrose and etiolation. Additionally, induction of AtCOX6b-1 by GA3 and AtCOX6b-3 by 6-benzylaminopurine was observed. Site II elements (TGGGCC/T) present in the three promoters bind common nuclear proteins and are important for basal and induced expression. Induction by sucrose requires, in addition, the integrity of elements with the sequence TACTAA. The results imply the participation of common regulatory factors in the expression of the three Arabidopsis COX6b genes.

Keywords: Arabidopsis thaliana - COX6b gene - Gene expression - Promoter analysis - Site II element - Sucrose-responsive element

Abbreviations: BAP, 6-benzylaminopurine; COX, cyto-chrome c oxidase; EMSA, electrophoretic mobility shift assay; GUS, β-glucuronidase.

(Received May 20, 2009; Accepted May 31, 2009)
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