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Plant and Cell Physiology Advance Access originally published online on May 13, 2009
Plant and Cell Physiology 2009 50(7):1382-1386; doi:10.1093/pcp/pcp069
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

This article appears in the following Plant and Cell Physiology issue: Special Issue Articles: Omics and Bioinformatics [View the issue table of contents]

Short Communication

Arabidopsis Mutants Carrying Chimeric Sigma Factor Genes Reveal Regulatory Determinants for Plastid Gene Expression

Jennifer Schweer1, Simon Geimer2, Jörg Meurer2 and Gerhard Link1,*

1Plant Cell Physiology, University of Bochum, D-44780 Bochum, Germany
2Botany Department, Biocenter, Ludwig-Maximilians-University Munich, D-82152 Planegg-Martinsried, Germany

*Corresponding author: E-mail, gerhard.link{at}ruhr-uni-bochum.de; Fax, +49-234-321-4188.


  Abstract

Like bacteria, plastids contain sigma factors for promoter binding and transcription initiation. Accumulating evidence suggests that members of the plant sigma factor family can have specialized non-redundant roles in terms of promoter preference in various developmental and environmental situations. To specify regulatory determinants, we have chosen pairwise exchange of portions of Arabidopsis sigma coding regions, followed by transformation of the chimeric constructs into a sigma 6 knockout line. The resulting phenotypes and plastid RNA patterns point to an important though not exclusive role for the highly variable N-terminal portion of plant sigma proteins.

Keywords: Arabidopsis - Chloroplast transcription - Chimeric sigma factors - Domain swap experiments - Regulatory determinants

Abbreviations: CaMV, cauliflower mosaic virus; CR, ‘conserved’ C-terminal region; GABI-Kat, German Plant Genomics Program ‘Genome Analysis in Biological Systems-Knockout Arabidopsis thaliana’; NEP, nucleus-encoded polymerase, a single-subunit phage-type DNA-dependent RNA polymerase; PEP, plastid-encoded polymerase, a multisubunit bacterial-type RNA polymerase; SIG, transcription initiation factor sigma; sig, sigma knockout line; UCR, ‘unconserved’ N-terminal region; WT, wild type.

This paper is dedicated to Professor Achim Trebst on the occasion of his 80th birthday.

(Received March 6, 2009; Accepted May 10, 2009)
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