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Plant and Cell Physiology Advance Access originally published online on April 30, 2009
Plant and Cell Physiology 2009 50(6):1127-1141; doi:10.1093/pcp/pcp064
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

A SUPERMAN-like Gene is Exclusively Expressed in Female Flowers of the Dioecious Plant Silene latifolia

Yusuke Kazama1,*, Makoto T. Fujiwara1,3, Ayako Koizumi2, Kiyoshi Nishihara2, Rie Nishiyama2,4, Etsuko Kifune2,5, Tomoko Abe1 and Shigeyuki Kawano2

1RIKEN Nishina Center, 2-1 Hirosawa, Wako, Saitama, 351-0198 Japan
2Department of Integrated Sciences, Graduate School of Frontier Sciences, University of Tokyo, FSB-601, 5-1-5 Kashiwanoha, Kashiwa, Chiba, 277-8562 Japan

*Corresponding author: E-mail, ykaze{at}riken.jp; Fax, +81-48-462-4674.


  Abstract

To elucidate the mechanism(s) underlying dioecious flower development, the present study analyzed a SUPERMAN (SUP) homolog, SlSUP, which was identified in Silene latifolia. The sex of this plant is determined by heteromorphic X and Y sex chromosomes. It was revealed that SlSUP is a single-copy autosomal gene expressed exclusively in female flowers. Introduction of a genomic copy of SlSUP into the Arabidopsis thaliana sup (sup-2) mutant complemented the excess-stamen and infertile phenotypes of sup-2, and the overexpression of SlSUP in transgenic Arabidopsis plants resulted in reduced stamen numbers as well as the suppression of petal elongation. During the development of the female flower in S. latifolia, the expression of SlSUP is first detectable in whorls 2 and 3 when the normal expression pattern of the B-class flowering genes was already established and persisted in the stamen primordia until the ovule had matured completely. In addition, significant expression of SlSUP was detected in the ovules, suggestive of the involvement of this gene in ovule development. Furthermore, it was revealed that the de-suppression of stamen development by infection of the S. latifolia female flower with Microbotryum violaceum was accompanied by a significant reduction in SlSUP transcript levels in the induced organs. Taken together, these results demonstrate that SlSUP is a female flower-specific gene and suggest that SlSUP has a positive role in the female flower developmental pathways of S. latifolia.

Keywords: Dioecious plant - Female flower specific expression - Floral development - Silene latifolia - SUPERMAN

Abbreviations: CaMV, Cauliflower mosaic virus; NJ, neighbor-joining; RT-PCR, reverse transcription PCR; QRT-PCR, quantitative reverse transcription-PCR; RACE, Rapid Amplification of cDNA Ends; SUP, SUPERMAN; TFIIIA, transcription factor IIIA; TSA, tyramide signal amplification

3Present address: Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba 3-8-1, Meguro, Tokyo, 153-8902 Japan.

4Present address: RIKEN Plant Science Center, Yokohama, Kanagawa, 203-0045 Japan.

5Present address: Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo 7-3-1, Bunkyo, Tokyo, 113-0033 Japan.

The nucleotide sequence reported in this paper has been submitted to DDBJ database under accession number AB246393 [GenBank] (SlSUP).

(Received February 4, 2009; Accepted April 28, 2009)
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