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Plant and Cell Physiology Advance Access originally published online on January 16, 2009
Plant and Cell Physiology 2009 50(3):658-664; doi:10.1093/pcp/pcp009
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

Mitogen-Activated Protein Kinase Cascade in the Signaling for Polyamine Biosynthesis in Tobacco

Eun-Kyoung Jang1, Kwang-Hyun Min1, Su-Hyun Kim1, Seung-Hee Nam2, Shuqun Zhang3, Young Cheol Kim1, Baik Ho Cho1 and Kwang-Yeol Yang1,*

1Agricultural Plant Stress Research Center, Department of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea
2Jeonnam Agricultural Research and Extension Services, Jeonnam, Naju, 520-715, Republic of Korea
3Department of Biochemistry and Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA

*Corresponding author: E-mail, kyyang{at}chonnam.ac.kr; Fax, +82-62-530-0207.


  Abstract

Expression of NtNEK2DD, a constitutively active mutant of NtMEK2, activates endogenous salicylic acid-induced protein kinase (SIPK) and wounding-induced protein kinase (WIPK), and leads to several stress/defense responses in tobacco. In this study, we used ACP (annealing control primer)-based differential display reverse transcription–PCR to isolate the downstream effectors mediated by the NtMEK2–SIPK/WIPK cascade. The arginine decarboxylase gene (ADC), which is involved in plant putrescine biosynthesis, was one of nine differentially expressed genes. When compared with NtMEK2KR plants, NtMEK2DD transgenic plants exhibited a significant increase in ADC and ODC (ornithine decarboxylase) transcript levels, as well as in putrescine and its catabolite, {gamma}-aminobutyric acid, following SIPK/WIPK activation. Taken together, these results suggest that the NtMEK2–SIPK/WIPK cascade is involved in regulating polyamine synthesis, especially putrescine synthesis, through transcriptional regulation of the biosynthetic genes in tobacco.

Keywords: Arginine decarboxylase - ACP-based DDRT-PCR - NtMEK2–SIPK/WIPK cascade - Polyamine biosynthesis.

Abbreviations: ACP, annealing control primer; ADC, arginine decarboxylase; DAO, diamine oxidase; DDRT–PCR, differential display reverse transcription-polymerase chain reaction; DEG, differentially expressed gene; DEX, dexamethasone; GABA, {gamma}-aminobutyric acid; HR, hypersensitive response; MAPK, mitogen-activated protein kinase; ODC, ornithine decarboxylase; PA, polyamine; PAO, polyamine oxidase; Put, putrescine; SA, salicylic acid; SIPK, salicylic acid-induced protein kinase; Spd, spermidine; SPDS, spermidine synthase; Spm, spermine; TLC, thin-layer chromatography; TMV, tobacco mosaic virus; WIPK, wounding-induced protein kinase.

(Received October 15, 2008; Accepted January 13, 2009)
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