Skip Navigation


Plant and Cell Physiology Advance Access originally published online on January 30, 2009
Plant and Cell Physiology 2009 50(3):554-571; doi:10.1093/pcp/pcp015
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Data
Right arrow All Versions of this Article:
50/3/554    most recent
pcp015v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Lewis, D.
Right arrow Articles by Newbigin, E. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lewis, D.
Right arrow Articles by Newbigin, E. J.
Agricola
Right arrow Articles by Lewis, D.
Right arrow Articles by Newbigin, E. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Aberrant Cell Expansion in the elongation Mutants of Barley

Dyani Lewis1,3, Antony Bacic1, Peter M. Chandler2 and Edward J. Newbigin1,*

1Plant Cell Biology Research Centre and Australian Centre for Plant Functional Genomics, School of Botany, University of Melbourne, Parkville, VIC, 3010, Australia
2CSIRO, Plant Industry, Canberra, ACT, 2601, Australia

*Corresponding author: E-mail, edwardjn{at}unimelb.edu.au; Fax, +61-3-9347-1071.


  Abstract

The elongation (elo) mutants of barley (Hordeum vulgare cv 'Himalaya') are a class of dwarf plants with defects affecting cell expansion. The phenotypes of mutants in three of the elo loci (elo1, elo2 and elo3) are recessive to the wild-type allele, and the mutations at elo-4 and elo-5 are semi-dominant. Allelism tests showed that elo1, elo2 and elo3 were at separate loci, and mapping data indicated that elo-5 was possibly allelic to either elo1 or elo2. A phenotype common to all elo mutants was the presence of short, radially swollen cells on the leaf epidermis, indicating a defect in longitudinal cell expansion. In three of the mutants, elo1, elo3 and elo5, this was accompanied by a twisting growth habit. Two of the mutations, elo2 and elo-5, affected cell division, with aberrant periclinal cell division resulting in the formation of increased cell layers in the leaf epidermis of elo2 and elo-5 homozygotes and in the aleurone layer of elo2 grains. Misplaced anticlinal divisions also occurred in the elo-5 leaf epidermis. Leaf cell walls of all elo lines contained less cellulose than the wild- type, and the cortical microtubules in elongating root epidermal cells in some elo lines were more randomly oriented than in the wild-type, consistent with the presence of radially swollen cells. We discuss possible functions for the Elo genes in primary cell wall synthesis.

Keywords: Barley - Cell expansion - Cell wall - Hordeum vulgare.

Abbreviations: BSA, bovine serum albumin; CESA, cellulose synthase; CSLF, CELLULOSE SYNTHASE-LIKE F; DCB, 2,6-dichlorobenzonitrile; GA, gibberellin; GA3, gibberellic acid; GAX, glucuronoarabinoxylan; HG, homogalacturonan; MLG, mixed-linkage (1->3), (1->4)-β-D-glucan; MT, microtubule; PBS, phosphate-buffered saline; RG, rhamnogalacturonan; SEM, scanning electron microscopy; SSR, simple sequence repeat; VIGS, virus-induced gene silencing; XG, xyloglucan

3Present address: School of Biological Sciences, Monash University, Clayton, VIC, 3800, Australia.

(Received December 15, 2008; Accepted January 23, 2009)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.