Skip Navigation


Plant and Cell Physiology Advance Access originally published online on January 28, 2009
Plant and Cell Physiology 2009 50(3):528-540; doi:10.1093/pcp/pcp013
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
50/3/528    most recent
pcp013v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Amenós, M.
Right arrow Articles by Barceló, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Amenós, M.
Right arrow Articles by Barceló, J.
Agricola
Right arrow Articles by Amenós, M.
Right arrow Articles by Barceló, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Different Effects of Aluminum on the Actin Cytoskeleton and Brefeldin A-Sensitive Vesicle Recycling in Root Apex Cells of Two Maize Varieties Differing in Root Elongation Rate and Aluminum Tolerance

Montse Amenós1,2, Isabel Corrales2, Charlotte Poschenrieder2, Peter Illés3, Frantisek Baluska1,3 and Juan Barceló2,*

1Institut für Zelluläre und Molekuläre Botanik, Universität Bonn, Kirschallee 1, D-53115 Bonn, Germany
2Laboratorio de Fisiología Vegetal, Facultad Biociencias, Universidad Autónoma de Barcelona, E-08193 Bellaterra, Spain
3Institute of Botany, Slovak Academy of Sciences, Dubravska cesta 14, SK-845 23, Bratislava, Slovakia

*Corresponding author: E-mail, juan.barcelo{at}uab.es: Fax, 34 935811321


  Abstract

A relationship between aluminum (Al) toxicity, endocytosis, endosomes and vesicle recycling in the root transition zone has recently been demonstrated. Here the importance of filamentous actin (F-actin)-based vesicle trafficking for Al tolerance has been investigating in maize varieties differing in their Al sensitivities. More Al was internalized into root tip cells of the Al-sensitive variety 16x36 than in the Al-tolerant variety Cateto. The actin cytoskeleton and vesicle trafficking were primary targets for Al toxicity in the root tips of the sensitive variety. Visualization of boron-cross-linked rhamnogalacturonan II (RGII)-containing brefeldin A (BFA) compartments revealed that Al inhibited the formation of these compartments, especially in variety 16x36. The time sequence of Al effects on pectin recycling matches the growth effects of Al in this sensitive variety. These results support the hypothesis that Al binding to pectin-rich cell walls can contribute to reversible inhibition of root elongation. Al-induced alterations on F-actin were most evident in the central part of the transition zone of Al-sensitive 16x36, where Al was localized inside the nucleoli. In relation to this observation, a role for symplastic Al in both irreversible growth inhibition and amelioration of BFA-induced inhibition of root elongation is discussed.

Keywords: Aluminum - Boron - Brefeldin A - Maize - Nucleolus - Vesicle trafficking.

Abbreviations: ARF, adenosyl ribosylation factor; BFA, brefeldin A; BSA, bovine serum albumin; DAPI, 4',6-diamidino-2-phenylindole; FITC, fluorescein isothiocyanate; GEF, guanine nucleotide exchange factor; PBS, phosphate-buffered saline; RGII, rhamnogalacturonan II.

(Received January 6, 2009; Accepted January 22, 2009)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.