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Plant and Cell Physiology Advance Access originally published online on July 1, 2008
Plant and Cell Physiology 2008 49(8):1256-1261; doi:10.1093/pcp/pcn098
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

Live Cell Imaging Reveals Plant Aurora Kinase Has Dual Roles During Mitosis

Daisuke Kurihara, Sachihiro Matsunaga, Susumu Uchiyama and Kiichi Fukui*

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871 Japan

*Corresponding author: E-mail, kfukui{at}bio.eng.osaka-u.ac.jp; Fax, +81-6-6879-7441.


  Abstract

The proper segregation of chromosomes during mitosis is required for accurate distribution of genetic information by two daughter cells. Here, we used live cell imaging of microtubules and kinetochores after treatment with an Aurora kinase inhibitor, hesperadin, in tobacco BY-2 cells to analyze the function of plant Aurora kinase during mitosis. Hesperadin treatment induced the delay of CenH3 alignment on the spindle equator. Furthermore, two types of dynamics of lagging CenH3s were observed during chromosome segregation. The findings indicate that the plant Aurora kinase has dual roles; correction of aberrant kinetochore–microtubule attachment and dissociation of cohesin during chromosome alignment and segregation.

Keywords: Chromosome segregation - Cohesin dissociation - Kinetochore–microtubule attachment - Lagging chromosome - Plant Aurora kinase

Abbreviations: ANOVA, analysis of variance; BY-2, Bright Yellow-2; BY-GTRC, BY-2 cells stably expressing GFP–{alpha}-tubulin–RFP–CenH3; CCA, complete CenH3 alignment; NEBD, nuclear envelope breakdown; RFP, red fluorescent protein.

(Received April 21, 2008; Accepted June 26, 2008)
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