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Plant and Cell Physiology Advance Access originally published online on June 5, 2008
Plant and Cell Physiology 2008 49(7):1128-1132; doi:10.1093/pcp/pcn088
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

Effects of Polylinker uATGs on the Function of Grass HKT1 Transporters Expressed in Yeast Cells

María A. Bañuelos1, Rosario Haro1, Ana Fraile-Escanciano and Alonso Rodríguez-Navarro*

Departamento de Biotecnología, Universidad Politécnica de Madrid, 28040 Madrid, Spain

*Corresponding author: E-mail, alonso.rodriguez{at}upm.es


  Abstract

HvHKT1 mediates K+ or Na+ uniport in yeast cells if the expression promoter is joined directly to the HvHKT1 cDNA, and Na+–K+ symport if a 59 nucleotide polylinker is inserted. Our results show that three ATG triplets in the polylinker decreased the synthesis of the transporter and that the lower amount of transporter caused the functional change. With the rice HKT1 cDNA, the 59 nt polylinker changed the mode of Na+ uptake from K+-insensitive to K+-inhibitable. These two modes of Na+ uptake also occurred in rice plants.

Keywords: Plant - Potassium - Sodium - Transport - uORFs - Yeast

Abbreviations: GFP, green fluorescent protein; ORF, open reading frame; uORF, upstream open reading frame.

1These authors contributed equally to this work.

(Received April 11, 2008; Accepted May 31, 2008)
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