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Plant and Cell Physiology Advance Access originally published online on May 29, 2008
Plant and Cell Physiology 2008 49(7):1122-1127; doi:10.1093/pcp/pcn080
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

RNAi-Mediated Knockdown of the XIP-Type Endoxylanase Inhibitor Gene, OsXIP, Has No Effect on Grain Development and Germination in Rice

Takaaki Tokunaga, Yoshie Miyata, Yukichi Fujikawa and Muneharu Esaka*

Graduate School of Biosphere Sciences, Hiroshima University, 1-4-4, Kagamiyama, Higashi-Hiroshima, 739-8528 Japan

*Corresponding author: E-mail, mesaka{at}hiroshima-u.ac.jp; Fax, +81-824-24-7927.


  Abstract

OsXIP (Oryza sativa xylanase inhibitor protein) is a XIP-type xylanase inhibitor which was identified as a protein encoded by a wound stress-responsive gene in rice. Although the OsXIP gene was specifically expressed in mature grains under basal conditions, recombinant OsXIP had no effect on rice endogenous xylanases, and OsXIP-suppressed transgenic rice plants did not exhibit any change in grain development and germination, suggesting that rice development may be independent of OsXIP. Analysis using an OsXIP-specific antibody revealed that OsXIP is markedly accumulated in apoplast in rice root cells by wounding. These results reinforced the possibility that OsXIP is involved in plant defense mechanisms against phytopathogens.

Keywords: Oryza sativa - Stress response - Xylanase inhibitor

Abbreviations: G6PDH, glucose-6-phosphate dehydrogenase; GH, glycoside hydrolase family; HPT, hygromycin resistance gene; IPTG, isopropyl-thio-β-D-galactoside; OsXIP, Oryza sativa xylanase inhibitor protein; RIXI, rice xylanase inhibitor protein; RNAi, RNA interference; RT–PCR, reverse transcription–PCR; TAXI, Triticum aestivum xylanase inhibitor; TLXI, thaumatin-like xylanase inhibitor; UTR, untranslated region; XIP, xylanase inhibitor protein.

(Received March 29, 2008; Accepted May 25, 2008)
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