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Plant and Cell Physiology Advance Access originally published online on May 28, 2008
Plant and Cell Physiology 2008 49(7):1056-1065; doi:10.1093/pcp/pcn081
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Enzymatic and Metabolic Diagnostic of Nitrogen Deficiency in Arabidopsis thaliana Wassileskija Accession

Thomas Lemaître1, Laure Gaufichon1, Stéphanie Boutet-Mercey, Aurélie Christ and Céline Masclaux-Daubresse*

Unité de Nutrition Azotée des Plantes, UNAP, UR511, INRA, Route de Saint Cyr, F-78000 Versailles, France

*Corresponding author: E-mail, masclaux{at}versailles.inra.fr; Fax, +33-1-30-83-30-96.


  Abstract

Adaptation to steady-state low-nutrient availability was investigated by comparing the Wassileskija (WS) accession of Arabidopsis thaliana grown on 2 or 10 mM nitrate. Low nitrogen conditions led to a limited rosette biomass and seed yield. The latter was mainly due to reduced seed number, while seed weight was less affected. However, harvest index was lower in high nitrate compared with limited nitrate conditions. Under nitrogen-limiting conditions, nitrate reductase activity was decreased while glutamine synthetase activity was increased due to a higher accumulation of the cytosolic enzyme. The level of nitrogen remobilization to the seeds was higher under low nitrogen, and the vegetative parts of the plants remaining after seed production stored very low residual nitrogen. Through promoting nitrogen remobilization and recycling pathways, nitrogen limitation modified plant and seed compositions. Rosette leaves contained more sugars and less free amino acids when grown under nitrogen-limiting conditions. Compared with high nitrogen, the levels of proline, asparagine and glutamine were decreased. The seed amino acid composition reflected that of the rosette leaves, thus suggesting that phloem loading for seed filling was poorly selective. The major finding of this report was that together with decreasing biomass and yield, nitrogen limitation triggers large modifications in vegetative products and seed quality.

Keywords: Asparagine - Glutamine synthetase - Nitrate reductase - Nitrogen limitation - Nitrogen remobilization - Seed composition

Abbreviations: DAS, days after sowing; GABA, {gamma}-aminobutyric acid; GS, glutamine synthetase; HN, high nitrogen nutrition; LN, low nitrogen nutrition; NHI, nitrgen harvest index; NR, nitrate reductase; NRE, nitrogen remobilization efficiency; %P, partitioning; RSA, relative specific allocation.

1These authors contributed equally to this work.

(Received March 28, 2008; Accepted May 24, 2008)
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