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Plant and Cell Physiology Advance Access originally published online on February 21, 2008
Plant and Cell Physiology 2008 49(4):570-582; doi:10.1093/pcp/pcn029
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Effects of Conditional IPT-Dependent Cytokinin Overproduction on Root Architecture of Arabidopsis Seedlings

Alena Kuderová1, Ivana Urbánková1, Martina Válková1, Jirí Malbeck2, Bretislav Brzobohaty1,3, Danka Némethová4,5 and Jan Hejátko1,*

1Laboratory of Plant Molecular Physiology, Department of Functional Genomics and Proteomics, Institute of Experimental Biology, Masaryk University, Kamenice 5, CZ-625 00, Brno, Czech Republic
2Institute of Experimental Botany, The Academy of Sciences of the Czech Republic V.V.i., Rozvojová 135, CZ-165 02 Prague, Czech Republic
3Institute of Biophysics, The Academy of Sciences of the Czech Republic V.V.i., Královopolská 135, CZ-612 65, Brno, Czech Republic
4Institute of Biostatistics and Analyses, Masaryk University, Kamenice 126/3, CZ-625 00 Brno, Czech Republic
5Research Centre for Environmental Chemistry and Ecotoxicology, Masaryk University, Kamenice 126/3, CZ-625 00, Brno, Czech Republic

*Corresponding author: E-mail, hejatko{at}sci.muni.cz; Fax, +420-5-4949-2640.


  Abstract

Cytokinin (CK) has been known to inhibit primary root elongation and suggested to act as an auxin antagonist in the regulation of lateral root (LR) formation. While the role of auxin in root development has been thoroughly studied, the detailed and overall description of CK effects on root system morphology, particularly that of developing lateral root primordia (LRPs), and hence its role in organogenesis is still in progress. Here we examine the effects of conditional endogenous CK overproduction on root architecture and consider its temporal aspect during the early development of Arabidopsis thaliana. We employed the pOp/LhGR system to induce ectopic ipt overexpression with a glucocorticoid dexamethasone at designated developmental points. The transient CaMV 35S>GR>ipt transactivation greatly enhanced levels of biologically active CKs of zeatin (Z)-type and identified a distinct developmental interval during which primary root elongation is susceptible to increases in endogenous CK production. Long-term CK overproduction inhibited primary root elongation by reducing quantitative parameters of primary root meristem, disturbed a characteristic graded distribution pattern of auxin response in LRPs and impaired their development. Our findings indicate the impact of perturbed endogenous CK on the regulation of asymmetric auxin distribution during LRP development and imply that there is cross-talk between auxin and CK during organogenesis in A. thaliana.

Keywords: Arabidopsis thaliana - Auxin–cytokinin cross-talk - CaMV 35S>GR>ipt transactivation - DR5 activity - Endogenous cytokinin - Root development

Abbreviations: AHK, Arabidopsis thaliana histidine kinase; CaMV, cauliflower mosaic virus; CK, cytokinin; DAG, days after germination; DEX, dexamethasone; GFP, green fluorescent protein; GR LBD, glucocorticoid ligand-binding domain; GUS, β-glucuronidase; HSP, heat-shock protein; iP, isopentenyl adenine; IPT, isopentenyltransferase; LR, lateral root; LRP, lateral root primordium; Z, zeatin; ZRP, zeatin riboside phosphate.

(Received December 1, 2007; Accepted February 18, 2008)
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