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Plant and Cell Physiology Advance Access originally published online on February 16, 2008
Plant and Cell Physiology 2008 49(4):512-525; doi:10.1093/pcp/pcn025
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

SodERF3, a Novel Sugarcane Ethylene Responsive Factor (ERF), Enhances Salt and Drought Tolerance when Overexpressed in Tobacco Plants

L. E. Trujillo1,*, M. Sotolongo1, C. Menéndez1, M. E. Ochogavía1, Y. Coll1, I. Hernández2, O. Borrás-Hidalgo2, B. P. H. J. Thomma3, P. Vera4 and L. Hernández1

1Laboratory of Plant Microbe Interactions, Center for Genetic Engineering and Biotechnology, Havana, Cuba
2Laboratory of Functional Genomics, Center for Genetic Engineering and Biotechnology, Havana, Cuba
3Laboratory of Phytopathology, Wageningen University, Binnenhaven 5, 6709 PD Wageningen, The Netherlands
4Laboratory of Plant Response to Biotic/Abiotic Factors, Institute of Molecular and Cellular Plant Biology (IBMCP), Valencia, Spain

*Corresponding author: E-mail, luis.trujillo{at}cigb.edu.cu; Fax, +53-7-331-779.


  Abstract

The molecular signals and pathways that govern biotic and abiotic stress responses in sugarcane are poorly understood. Here we describe SodERF3, a sugarcane (Saccharum officinarum L. cv Ja60-5) cDNA that encodes a 201-amino acid DNA-binding protein that acts as a transcriptional regulator of the ethylene responsive factor (ERF) superfamily. Like other ERF transcription factors, the SodERF3 protein binds to the GCC box, and its deduced amino acid sequence contains an N-terminal putative nuclear localization signal (NLS). In addition, a C-terminal short hydrophobic region that is highly homologous to an ERF-associated amphiphilic repression-like motif, typical for class II ERFs, was found. Northern and Western blot analysis showed that SodERF3 is induced by ethylene. In addition, SodERF3 is induced by ABA, salt stress and wounding. Greenhouse-grown transgenic tobacco plants (Nicotiana tabacum L. cv. SR1) expressing SodERF3 were found to display increased tolerance to drought and osmotic stress.

Keywords: Abiotic stress - ABA - Ethylene responsive factors - EAR motif - Salt and drought tolerance - Sugarcane

Abbreviations: AP2, APETALA 2; CaMV, cauliflower mosaic virus; CRT, C-repeat; DRE, dehydration-responsive element; EAR, ERF-associated amphiphilic repression; EMSA, electrophoretic mobility shift assay; ERF, ethylene responsive factor; EST, expressed sequence tag; NLS, nuclear localization signal; SA, salicylic acid; TF, transcription factor

(Received November 17, 2007; Accepted February 12, 2008)
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