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Plant and Cell Physiology Advance Access originally published online on January 4, 2008
Plant and Cell Physiology 2008 49(2):284-289; doi:10.1093/pcp/pcm182
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

A Novel orf108 Co-Transcribed with the atpA Gene is Associated with Cytoplasmic Male Sterility in Brassica juncea Carrying Moricandia arvensis Cytoplasm

Ashutosh1,4, Pankaj Kumar1,4, V. Dinesh Kumar2, Prakash C. Sharma3, Shyam Prakash1 and Shripad R. Bhat1,*

1National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi-110012, India
2Directorate of Oilseeds Research, Rajendranagar, Hyderabad-500030, India
3Guru Gobind Singh Indraprastha University, Kashmere Gate, Delhi-110006, India

*Corresponding author: E-mail, srbhat22{at}rediffmail.com; Fax, +91-11-25843984.


  Abstract

Mitochondrial atpA transcripts were examined in cytoplasmic male sterile (CMS) and fertility restorer lines of CMS (Moricandia arvensis) Brassica juncea. Male sterile flowers had longer atpA transcripts than male fertiles. The mitochondrial atpA region of the CMS line was cloned and sequenced. The 5' and 3' ends of the atpA transcripts of the CMS and the fertility restorer lines were mapped and full-length transcripts were cloned and sequenced. A novel orf108 (open reading frame 108) co-transcribed with the atpA gene was found in the male sterile flowers. In the fertility restorer line, the transcript was cleaved within orf108 to yield monocistronic atpA transcripts.

Keywords: atpA - Brassica juncea - Cytoplasmic male sterility - Fertility restoration - orf108 - Transcript mapping

Abbreviations: cRT–PCR, circular reverse transcription–PCR; CMS, cytoplasmic male sterility; ORF, open reading frame; TAP, tobacco acid pyrophosphatase.

4These authors contributed equally to this work.

(Received October 8, 2007; Accepted December 22, 2007)
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