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Plant and Cell Physiology Advance Access originally published online on October 11, 2008
Plant and Cell Physiology 2008 49(11):1758-1763; doi:10.1093/pcp/pcn149
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

Degradation of Sphingoid Long-Chain Base 1-Phosphates (LCB-1Ps): Functional Characterization and Expression of AtDPL1 Encoding LCB-1P Lyase Involved in the Dehydration Stress Response in Arabidopsis

Masahiro Nishikawa1, Kenta Hosokawa1, Mai Ishiguro1, Hiroki Minamioka1, Kentaro Tamura2, Ikuko Hara-Nishimura2, Yohei Takahashi3, Ken-ichiro Shimazaki3 and Hiroyuki Imai1,*

1 Department of Biology, Graduate School of Natural Science, Konan University, Kobe, 658-8501 Japan
2 Department of Botany, Graduate School of Science, Kyoto University, Kyoto, 606-8502 Japan
3 Department of Biology, Faculty of Science, Kyushu University, Fukuoka, 810-8560 Japan

*Corresponding author: E-mail, imai{at}konan-u.ac.jp; Fax, +81-78-4352539.


  Abstract

Sphingoid long-chain base (LCB) 1-phosphates are degradated by LCB 1-phosphate lyase to C16 fatty aldehydes and phosphoethanolamine. Here, we confirmed that the At1g27980 gene product, AtDPL1, is a functional LCB-1-phosphate lyase. Expression of green fluorescent protein fusion products in suspension-cultured Arabidopsis cells showed that AtDPL1 is located to the endoplasmic reticulum. The rates of fresh weight decreases of dpl1-1 and dpl1-2 mutants were significantly slower than those of the wild-type plants. This ability to limit their transpiration reflected the leaf temperature of the mutant plants more than that of wild-type plants, suggesting that AtDPL1 plays a role in dehydration stress.

Keywords: Dehydration stress - Long-chain base - Sphingosine-1-phosphate lyase - Transpiration

Abbreviations: CaMV, cauliflower mosaic virus; DPL, dihydrosphingosine-1-phosphate lyase; ER, endoplasmic reticulum; GFP, green fluorescent protein; GUS, β-glucuronidase; LCB, sphingoid long-chain base; LCB-1P, sphingoid long-chain base 1-phosphate; ORF, open reading frame; PHS, (t18:0) phytosphingosine (4-hydroxysphingenine); RNAi, RNA interference; RT–PCR, reverse transcription–PCR; SPH, sphingosine (4E-sphingenine); SPH-1P, sphingosine 1-phosphate; SPL, sphingosine 1-phosphate lyase.

(Received July 16, 2008; Accepted October 6, 2008)
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