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Plant and Cell Physiology Advance Access originally published online on August 20, 2008
Plant and Cell Physiology 2008 49(10):1429-1450; doi:10.1093/pcp/pcn123
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and the Japanese Society of Plant Physiologists are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Special Issue - Regular Paper

Comprehensive Transcriptome Analysis of Phytohormone Biosynthesis and Signaling Genes in Microspore/Pollen and Tapetum of Rice

Ko Hirano1,3, Koichiro Aya1,3, Tokunori Hobo1, Hitoshi Sakakibara2, Mikiko Kojima2, Rosalyn Angeles Shim1, Yasuko Hasegawa1, Miyako Ueguchi-Tanaka1 and Makoto Matsuoka1,*

1Bioscience and Biotechnology Center, Nagoya University, Nagoya, 464-8601 Japan
2RIKEN Plant Science Center, Tsurumi, Yokohama, 230-0045 Japan

*Corresponding author: E-mail, makoto{at}agr.nagoya-u.ac.jp; Fax, +81-52-789-5226.


  Abstract

To investigate the involvement of phytohormones during rice microspore/pollen (MS/POL) development, endogenous levels of IAA, gibberellins (GAs), cytokinins (CKs) and abscisic acid (ABA) in the mature anther were analyzed. We also analyzed the global expression profiles of genes related to seven phytohormones, namely auxin, GAs, CKs, brassinosteroids, ethylene, ABA and jasmonic acids, in MS/POL and tapetum (TAP) using a 44K microarray combined with a laser microdissection technique (LM-array analysis). IAA and GA4 accumulated in a much higher amount in the mature anther compared with the other tissues, while CKs and ABA did not. LM-array analysis revealed that sets of genes required for IAA and GA synthesis were coordinately expressed during the later stages of MS/POL development, suggesting that these genes are responsible for the massive accumulation of IAA and GA4 in the mature anther. In contrast, genes for GA signaling were preferentially expressed during the early developmental stages of MS/POL and throughout TAP development, while their expression was down-regulated at the later stages of MS/POL development. In the case of auxin signaling genes, such mirror-imaged expression observed in GA synthesis and signaling genes was not observed. IAA receptor genes were mostly expressed during the late stages of MS/POL development, and various sets of AUX/IAA and ARF genes were expressed during the different stages of MS/POL or TAP development. Such cell type-specific expression profiles of phytohormone biosynthesis and signaling genes demonstrate the validity and importance of analyzing the expression of phytohormone-related genes in individual cell types independently of other cells/tissues.

Keywords: Anther - Auxin - GA - Laser microdissection - Microarray - Rice

Abbreviations: BC, bicellular POL stage; BR, brassinosteroid; CK, cytokinin; GA, gibberellin; JA, jasmonic acid; MEI, meiosis; MS, microspore; PCD, programmed cell death; POL, pollen; SI, signal intensity; TAP, tapetum; TC, tricellular POL stage; TET, tetrad stage; UN, uninuclear MS stage

3These authors contributed equally to this work.

(Received August 6, 2008; Accepted August 18, 2008)
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