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Plant and Cell Physiology Advance Access originally published online on November 23, 2007
Plant and Cell Physiology 2008 49(1):30-39; doi:10.1093/pcp/pcm162
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Tissue and Cell-Specific Localization of Rice Aquaporins and Their Water Transport Activities

Junko Sakurai1,2,*, Arifa Ahamed1, Mari Murai1, Masayoshi Maeshima2 and Matsuo Uemura3

1Climate Change Research Team, National Agricultural Research Center for Tohoku Region, Morioka, 020-0198 Japan
2Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601 Japan
3United Graduate School of Agricultural Sciences and Cryobiosystem Research Center, Faculty of Agriculture, Iwate University, Morioka, 020-8550 Japan

*Corresponding author: E-mail, junkoi{at}affrc.go.jp; Fax, +81-19-641-7794.


  Abstract

Water transport in plants is greatly dependent on the expression and activity of water transport channels, called aquaporins. Here, we have clarified the tissue- and cell-specific localization of aquaporins in rice plants by immunoblotting and immunocytochemistry using seven isoform-specific aquaporin antibodies. We also examined water transport activities of typical aquaporin family members using a yeast expression system in combination with a stopped-flow spectrophotometry assay. OsPIP1 members, OsPIP2;1, OsTIP1;1 and OsTIP2;2 were expressed in both leaf blades and roots, while OsPIP2;3, OsPIP2;5 and OsTIP2;1 were expressed only in roots. In roots, large amounts of aquaporins accumulated in the region adjacent to the root tip (around 1.5–4 mm from the root tip). In this region, cell-specific localization of the various aquaporin members was observed. OsPIP1 members and OsTIP2;2 accumulated predominantly in the endodermis and the central cylinder, respectively. OsTIP1;1 showed specific localization in the rhizodermis and exodermis. OsPIP2;1, OsPIP2;3 and OsPIP2;5 accumulated in all root cells, but they showed higher levels of accumulation in endodermis than other cells. In the region at 35 mm from the root tip, where aerenchyma develops, aquaporins accumulated at low levels. In leaf blades, OsPIP1 members and OsPIP2;1 were localized mainly in mesophyll cells. OsPIP2;1, OsPIP2;3, OsPIP2;5 and OsTIP2;2 expressed in yeast showed high water transport activities. These results suggest that rice aquaporins with various water transport activities may play distinct roles in facilitating water flux and maintaining the water potential in different tissues and cells.

Keywords: Aquaporin - Cell-specific localization - Rice - Water transport activity

Abbreviations: NIP, nodulin 26-like intrinsic protein; PIP, plasma membrane intrinsic protein; RT–PCR, reverse transcription–PCR; SIP, small and basic intrinsic protein; TIP, tonoplast intrinsic protein.

(Received August 30, 2007; Accepted November 16, 2007)
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