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Plant and Cell Physiology Advance Access originally published online on December 15, 2007
Plant and Cell Physiology 2008 49(1):2-10; doi:10.1093/pcp/pcm174
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Rapid Paper

Disruption of a Gene Encoding C4-Dicarboxylate Transporter-Like Protein Increases Ozone Sensitivity Through Deregulation of the Stomatal Response in Arabidopsis thaliana

Shoko Saji1,2,6, Srinivas Bathula1,6,7, Akihiro Kubo1,6,*, Masanori Tamaoki1, Machi Kanna1,3,8, Mitsuko Aono1, Nobuyoshi Nakajima1, Tatsuro Nakaji4, Tomomi Takeda1, Munehiko Asayama2,5 and Hikaru Saji1

1Environmental Biology Division, National Institute for Environmental Studies, Tsukuba, Ibaraki, 305-8506 Japan
2United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509 Japan
3Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Kanagawa, 226-8501 Japan
4Center for Global Environmental Research (CGER), National Institute for Environmental Studies, Tsukuba, Ibaraki, 305-8506 Japan
5College of Agriculture, Ibaraki University, Ami, Inashiki, Ibaraki, 300-0393 Japan

*Corresponding author: E-mail, kub{at}nies.go.jp; Fax, +81-29-850-2391.


  Abstract

To understand better the plant response to ozone, we isolated and characterized an ozone-sensitive (ozs1) mutant strain from a set of T-DNA-tagged Arabidopsis thaliana ecotype Columbia. The mutant plants show enhanced sensitivity to ozone, desiccation and sulfur dioxide, but have normal sensitivity to hydrogen peroxide, low temperature and high light levels. The T-DNA was inserted at a single locus which is linked to ozone sensitivity. Identification of the genomic sequences flanking the T-DNA insertion revealed disruption of a gene encoding a transporter-like protein of the tellurite resistance/C4-dicarboxylate transporter family. Plants with either of two different T-DNA insertions in this gene were also sensitive to ozone, and these plants failed to complement ozs1. Transpiration levels, stomatal conductance levels and the size of stomatal apertures were greater in ozs1 mutant plants than in the wild type. The stomatal apertures of ozs1 mutant plants responded to light fluctuations but were always larger than those of the wild-type plants under the same conditions. The stomata of the mutant and wild-type plants responded similarly to stimuli such as light, abscisic acid, high concentrations of carbon dioxide and ozone. These results suggest that OZS1 helps to close stomata, being not involved in the responses to these signals.

Keywords: Arabidopsis thaliana - Mutant - Oxidative stress - Ozone - Stomata - Transporter

Abbreviations: JA, jasmonic acid; PCD, programmed cell death; ROS, reactive oxygen species; RT–PCR, reverse transcription–PCR; SA, salicylic acid; TAIL-PCR, thermal asymmetric interlaced PCR; TDT family, tellurite resistance/C4-dicarboxylate transporter family.

6These authors contributed equally to this work.

7Present address: CSN Degree College & PG Courses, Bhimavaram-3 Industrial Estate, W.G.Dt., Andhrapradesh, India.

8Present address: Graduate School of Comprehensive Scientific Research, Prefectural University of Hiroshima, Hiroshima, Hiroshima, 727-0023 Japan.

(Received November 1, 2007; Accepted December 6, 2007)
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